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This Week in PNAS - a Featured ArticleUnusual mitochondrial ribosome from a parasiteComplexes of proteins and RNA known as ribosomes play the crucial task of translating genetic messages, in the form of mRNAs, into proteins. Ubiquitous in eubacterial, eukaryotic cells, in the archaea, and in organelles, these workhorses of the genetic information system differ markedly in composition among the phylogenetic domains. Manjuli Sharma et al. found that the mitochondrial ribosome of the Leishmania tarentolae (Lmr) parasite contains several features previously unseen in cytoplasmic or organellar ribosomes. The Leishmania parasites, which are transmitted by the bite of sand flies, cause skin sores or potentially fatal visceral disease. The authors obtained a 3D, cryo-electron microscopic map of the Lmr parasite and discovered that, in addition to having lower RNA content, this mitochondrial ribosome was more porous than other known ribosome structures. Major portions of key functionally conserved sites, such as the channel where mRNA enters, the transfer RNA passage, and the region where nascent polypeptides exit, contain Lmr-specific proteins, rather than familiar ones. Despite the specificity in these regions, the overall architecture of the Lmr has been conserved, the authors say, suggesting the imposition of tight constraints on this evolutionarily divergent ribosomal form.  Structure of a mitochondrial ribosome with minimal RNA by Manjuli R. Sharma, Timothy M. Booth, Larry Simpson, Dmitri A. Maslov, and Rajendra K. Agrawal (see pages 9637-9642)
1. Simpson, L., Kirby-Smith, J. and Randolph, M.,
"Electron spin resonance in photodynamic dyes.", Nature 199, 243-245
(1963) 1966 2. Simpson, L., "Toxic impurities in Nalgene filter
units.", Science 153, 548 (1966)
1967 3. Simpson, L., "Morphogenesis and the function of the
kinetoplast in Leishmania.", Atlas de Symposia sobre a Biota Amazonica
(Pathologia) 6, 231-234 (1967) 1968 4. Simpson, L., "Behavior of the kinetoplast of Leishmania
tarentolae.", J. Protozool. 15, 132-136 (1968)
5. Simpson, L., "The leishmania-leptomonad transformation
of Leishmania donovani: nutritional requirements, respiration changes
and antigenic changes.", J. Protozool. 15, 201-207 (1968) 6. Simpson, L., "The effects of acriflavin on the
kinetoplast DNA of Leishmania tarentolae: mechanism of action and
physiological correlates of the loss of kinetoplast DNA.", J. Cell Biol.
37, 660-682 (1968) 1970 7. Simpson, L. and Braly, P., "Synchronization of
Leishmania tarentolae by hydroxyurea.", J. Protozool. 17, 511-517 (1970)
1971 8. Simpson, L. and da Silva, A., "Isolation and
characterization of kinetoplast DNA from Leishmania tarentolae. ", J.
Mol. Biol. 56, 443-473 (1971) 1972 9. Simpson, L., "The kinetoplast of the hemoflagellates.",
Intern. Rev. Cytol. 32, 139-207 (1972)
1973 10. Simpson, L., "Structure and function of the
kinetoplast DNA.", J. Protozool. 20, 2-8 (1973)
11. Wesley, R.D. and Simpson, L., "Studies on kinetoplast
DNA. I. Isolation of kinetoplast DNA minicircles from Leishmania
tarentolae.", Biochim. Biophys. Acta 319, 237-253 (1973) 12. Wesley, R.D. and Simpson, L., "Studies on kinetoplast
DNA. II. Biophysical properties of minicircular DNA from Leishmania
tarentolae.", Biochim. Biophys. Acta 319, 254-266 (1973)
13. Wesley, R.D. and Simpson, L., "Studies on kinetoplast
DNA. III. Kinetic complexity of kinetoplast and nuclear DNA from
Leishmania tarentolae.", Biochim. Biophys. Acta 319, 267-276
(1973)
14. Salser, W., Fry, K., Wesley, R.D. and Simpson, L.,
"Use of nucleic acid fingerprints to estimate the complexity of
minicircle DNA.", Biochim. Biophys. Acta 319, 277-280 (1973) 1974 15. Simpson, A. and Simpson, L., "Labeling of Crithidia
fasciculata DNA with 3H-thymidine.", J. Protozool. 21, 379-382 (1974)
16. Simpson, L. and Berliner, J., "Isolation of the
kinetoplast DNA of Leishmania tarentolae in the form of a network.", J.
Protozool. 21, 382-393 (1974) (PDF)
17. Simpson, L., Simpson, A. and Wesley, R., "Replication
of the kinetoplast DNA of Leishmania tarentolae and Crithidia
fasciculata.", Biochim. Biophys. Acta 349, 161-172 (1974)
18. Braly, P., Simpson, L. and Kretzer, F., "Isolation of
kinetoplast-mitochondrial complexes from Leishmania tarentolae.", J.
Protozool. 21, 782-790 (1974)
19. Simpson, L. and Simpson, A., "Isolation and
characterization of kinetoplast DNA networks from Crithidia
fasciculata.", J. Protozool. 21, 774-781 (1974)
20. Greenfield, L., Simpson, L. and Kaplan, D.,
"Conversion of closed circular DNA molecules to single-nicked molecules
by digestion with DNAse I in the presence of ethidium bromide.",
Biochim. Biophys. Acta. 407, 365-375 (1975)
1976 21. Simpson, A. and Simpson, L., "Pulse-labeling of
kinetoplast DNA: Localization of two sites of synthesis within the
networks and kinetics of labeling of closed minicircles.", J. Protozool.
23, 583-587 (1976)
22. Simpson, L. and Hyman, B., "Restriction enzyme
analysis of Leishmania tarentolae kinetoplast DNA.", in Biochemistry of
Parasites and Host-Parasite Relationships (H. Van den Bosche, ed.)
North-Holland Pub. Co., Amsterdam, (1976)
23. Simpson, L., Simpson, A. and Lasky, L., "Kinetoplast
RNA from Leishmania tarentolae culture forms.", in Biochemistry of
Parasites and Host-Parasite Relationships (H. Van den Bosche, ed.) North
Holland Pub. Co. Amsterdam, (1976) 1977 24. Brunk, C. and Simpson, L., "Comparison of various
ultraviolet sources for fluorescent detection of ethidium bromide-DNA
complexes in polyacrylamide gels.", Anal. Biochem. 82, 455-462 (1977)
1978 25. Simpson, L., "Glucose-sensitive strain of Trypanosoma
brucei.", J. Parasitol. 64, 360 (1978) 26. Simpson, L. and Simpson, A., "Kinetoplast RNA of
Leishmania tarentolae.", Cell 14, 169-178 (1978) 27. Cheng, D. and Simpson, L., "Isolation and
characterization of kinetoplast DNA and RNA of Phytomonas davidi.",
Plasmid 1, 279-315 (1978) 1979 28. Masuda, H., Simpson, L., Rosenblatt, H. and Simpson,
A., "Restriction map, partial cloning and localization of 9S and 12S RNA
genes on the maxicircle component of the kinetoplast DNA of Leishmania
tarentolae. ", Gene 6, 51-73 (1979) 29. Simpson, L., "Isolation of maxicircle component of
kinetoplast DNA from hemoflagellate protozoa.", Proc. Natl. Acad. Sci.
USA 76, 1585-1588 (1979)
30. Simpson, L., Simpson, A., Masuda, H., Rosenblatt, H.,
Michael, N. and Kidane, G., "Replication and transcription of
kinetoplast DNA", In Extrachromosomal DNA (Cummings, D., Borst, P.,
David, I., Weissman, J. and Fox, C. eds.) ICN-UCLA Symposium. XV,
533-548 (1979) 1980 31. Morel, C., Chiari, E., Camargo, E., Mattei, D.,
Romanha, A. and Simpson, L., "Strains and clones of Trypanosoma cruzi
can be characterized by restriction endonuclease fingerprinting of
kinetoplast DNA minicircles.", Proc. Natl. Acad. Sci. USA 77, 6810-681
(1980) 32. Simpson, L., Simpson, A., Kidane, G., Livingston, L.
and Spithill, T., "The kinetoplast DNA of the hemoflagellate protozoa.",
Am. J. Trop. Med. Hyg., Suppl. 29, 1053-1063 (1980)
33. Morel, C. and Simpson, L., "Characterization of
pathogenic trypanosomatidae by restriction endonuclease fingerprinting
of kinetoplast DNA minicircles.", Am. J. Trop. Med. Hyg. 29 Suppl.,
1070-1074 (1980)
34. Simpson,A. and Simpson, L., "Kinetoplast DNA and RNA
of Trypanosoma brucei. ", Mol. Biochem. Parasitol. 2, 93-108 (1980) 1981
35. Simpson, L. Morel, C. and Simpson, A., "Biological
role of extrachromosomal DNA.", In Modern Genetic Concepts and
Techniques in the Study of Parasites (F. Michal, ed.) Schwabe and Co. AG
Basel, 65-81 (1981) 1982 36. Simpson, L., Simpson, A., Spithill, T. and Livingston,
L., "Sequence organization of maxicircle kinetoplast DNA from Leishmania
tarentolae.", In Mitochondrial Genes (Borst, P., Attardi, G. and
Slonimski, P., eds.) Cold Spring Harbor Symposium, 435-439 (1982) 37. Hughes, D., Schneider, C. and Simpson, L.,
"Isolation and characterization of drug resistant mutants of Crithidia
fasciculata.", J. Parasitol. 68, 642-649 (1982)
38. Simpson, L., Simpson, A. and Livingston, L.,
"Transcription of the maxicircle kinetoplast DNA of Leishmania
tarentolae.", Mol. Biochem. Parasitol. 6, 237-257 (1982) 39. Simpson, L., Spithill,T. and Simpson, A.,
"Identification of maxicircle DNA sequences in Leishmania tarentolae
homologous to sequences of specific yeast mitochondrial structural
genes.", Mol. Biochem. Parasitol. 6, 253-263 (1982)
1983 40. Hughes, D., Simpson, L. and Schneider, C., "Flagellar
adherence of Crithidia fasciculata cells in culture.", J. Protozool. 30,
635-641 (1983)
41. Muhich, M., Simpson, L. and Simpson, A., "Comparison
of the maxicircle DNAs of Leishmania tarentolae and Trypanosoma
brucei.", Proc. Natl. Acad. Sci. USA 80, 4060-4064 (1983)
42. Spithill, T., Simpson, A.M. and Simpson, L.,
"Identification of sequence homologies between maxicircle DNA of
Leishmania tarentolae and specific yeast mitochondrial structural
genes.",In Manipulation and Expression of Genes in Eukaryotes (Nagley,
Linnane, Peacock and Pateman, eds.) Academic Press, Australia, 307-311
(1983)
43. Simpson, L., "The application of
recombinant DNA technology to problems of parasite and vector
identification.", In New Approaches to the Identification of Parasites
and Vectors (B. Newton and F. Michal, eds.) Schwabe AG, Basel, 71-95
(1984) 44. Kidane, G., Hughes, D. and Simpson, L., "Sequence
heterogeneity and anomalous electrophoretic mobility of kinetoplast
minicircle DNA in Leishmania tarentolae.", Gene 27, 265-277 (1984)
45. Hughes, D., Simpson, L., Kayne, P. and Neckelmann, N.,
"Autonomous replication sequences in the maxicircle kinetoplast DNA of
Leishmania tarentolae.", Mol. Biochem. Parasitol. 13, 263-275 (1984) 46. de la Cruz, V., Neckelmann, N. and Simpson, L.,
"Sequences of six structural genes and several open reading frames in
the kinetoplast maxicircle DNA of Leishmania tarentolae.", J. Biol.
Chem. 259, 15136-15147 (1984)
47. Morel, C., Goncalves, A., Simpson, L. and Simpson,
A.M., "Recent advances in the development of DNA hybridization probes
for the detection and characterization of Trypanosoma cruzi.", Mem.
Inst. Oswaldo Cruz Suppl. Vol. 79, 51-53 (1984)
48. de la Cruz, V.F., Lake, J.A., Simpson, A.M. and
Simpson, L., "A minimal ribosomal RNA: Sequence and secondary structure
of the 9S kinetoplast ribosomal RNA from Leishmania tarentolae.", Proc.
Natl. Acad. Sci. USA 82, 1401-1405 (1985)
49. de la Cruz, V., Simpson, A., Lake, J. and Simpson, L.,
"Primary sequence and partial secondary structure of the 12s kinetoplast
(mitochondrial) ribosomal RNA from Leishmania tarentolae: Conservation
of peptidyl- transferase structural elements.", Nucl. Acids Res. 13,
2337-2356 (1985)
50. Muhich, M., Neckelmann, N. and Simpson, L., "The
divergent region of the Leishmania tarentolae kinetoplast maxicircle DNA
contains a diverse set of repetitive sequences.", Nucl. Acids Res. 13,
3241-3260 (1985)
51. Simpson, A., Neckelmann, N., de la Cruz, V., Muhich,
M. and Simpson, L., "Mapping and 5' end determination of kinetoplast
maxicircle gene transcripts from Leishmania tarentolae.", Nucl.Acids
Res. 13, 5977-5993 (1985)
52. Simpson, A., Hughes, D. and Simpson, L. , "Trypanosoma
brucei: differentiation of in vitro-grown bloodstream trypomastigotes
into procyclic forms.", J. Protozool. 32 , 672-677 (1985)
53. Simpson, L., Simpson, A., de la Cruz, V., Neckelmann,
N. and Muhich, M., "Genomic organization and transcription of
mitochondrial maxicircle DNA in trypanosomid protozoa.", In Achievements
and Perspectives of Mitochodrial Research (Quagliarello, Slater,
Palmieri, Saccone and Kroon, Eds.) Vol. II, Elsevier Press, p. 99-110
(1985)
54. Hughes, D.E. and Simpson, L., "Introduction of plasmid
DNA into the trypanosomatid protozoan Crithidia fasciculata", Proc.
Natl. Acad. Sci. USA 83, 6058-6062 (1986)
55. Muhich, M. and Simpson, L., "Specific cleavage of
kinetoplast minicircle DNA from Leishmania tarentolae and identification
of several additional minicircle sequence classes", Nucl. Acids Res. 14,
5531-5556 (1986)
56. Simpson, L., "Kinetoplast DNA in trypanosomid
flagellates.", Intern. Rev. Cytol. 99, 119-179 (1986)
57. Dewes, H., Ostergaard, H. and Simpson, L., "Impaired
drug uptake in methotrexate resistant Crithidia fasciculata without
changes in dihydrofolate reductase activity or gene amplification." Mol.
Biochem. Parasitol. 19, 149-161 (1986)
58. Simpson, L., Neckelmann, N., de la Cruz, V., Simpson,
A., Feagin, J., Jasmer, D. and Stuart, K., "Comparison of the maxicircle
(mitochondrial) genomes of Leishmania tarentolae and Trypanosoma brucei
at the level of nucleotide sequence.", J. Biol. Chem. 262, 6182-6196
(1987)
59. Simpson, L., "The mitochondrial genome of
kinetoplastid protozoa: genomic organization, transcription, replication
and evolution.", Ann. Rev. Microbiol. 41, 363-382 (1987)
60. Muhich, M., Hughes, D., Simpson, A. and Simpson, L.,
"The monogenetic kinetoplastid protozoan, Crithidia fasciculata,
contains a transcriptionally active, multicopy mini-exon sequence",
Nucl. Acids Res. 15, 3141-3153 (1987)
61. Degrave, W., Fragoso, S., Britto, C., Van Heuverswyn,
H., Kidane, K., Cardoso, M., Mueller, R., Simpson, L. and Morel, C.,
"Peculiar sequence organization of kinetoplast DNA minicircles from
Trypanosoma cruzi", Mol. Biochem. Parasitol. 27, 63-70 (1988) 62. Gomez-Eichelmann, C., Holz, G., Beach, D., Simpson, A.
and Simpson, L., "Comparison of several lizard Leishmania species and
strains in terms of kinetoplast minicircle and maxicircle DNA sequences,
nuclear chromosomes and membrane lipids", Mol. Biochem. Parasitol. 27,
143-158 (1988) (PDF)
63. Feagin, J., Shaw, J., Simpson, L. and Stuart, K.,
"Creation of AUG initiation codons by addition of uridine within
cytochrome b transcripts of three kinetoplastid species." Proc. Natl.
Acad. Sci. USA. 85, 539-543 (1988) 64. Lake, J., , de la Cruz, V., Ferreira, P., Morel, C.
and Simpson, L., "Evolution of parasitism: Kinetoplastid protozoan
history reconstructed from mitochondrial rRNA sequences." Proc. Natl.
Acad. Sci. U.S.A. 85, 4779-4783 (1988)
65. Shaw, J., Feagin, J., Stuart, K. and Simpson, L.,
"Editing of kinetoplastid mitochondrial mRNAs by uridine addition and
deletion generates conserved amino acid sequences and AUG initiation
codons." Cell. 53, 401-411 (1988)
66. Simpson, L. and Holz, G., "The status of Leishmania
tarentolae/Trypanosoma platydactyli." Parasitol. Today 4, 115-118
(1988)
67. Simpson, L., "Gel electrophoresis and photography - An
application note." UVP Company applications note (1988) 68. Sturm, N., Simpson, L., Degrave, W. and Morel, C.,
"Sensitive detection and schizodeme classification of Trypanosoma cruzi
cells by amplification of kinetoplast minicircle DNA sequences: Use in
diagnosis of Chagas Disease." Mol. Biochem. Parasitol. 33, 205-214
(1989)
69. Hughes, D., Shonekan, O. and Simpson, L., "Structure,
organization and transcription of the bifunctional dihydrofolate
reductase-thymidylate synthetase gene from Crithidia fasciculata:
evidence of kinetoplastid transcriptional control signals." Mol.
Biochem. Parasitol. 34, 155-166 (1989)
70. Campbell, D.A., Spithill, T.W., Samaras, N., Simpson,
A.M. and Simpson, L., "Sequence of a cDNA for the ND1 gene from
Leishmania major: potential uridine addition in the polyadenosine tail."
Mol. Biochem. Parasitol. 36, 197-200 (1989)
71. Shaw, J. and Simpson, L., "Characterization of a
protein fraction containing cytochromes b and c1 from mitochondria of
Leishmania tarentolae." Exptl. Parasitol. 68, 443-449(1989) 72. Simpson, L. and Shaw, J., "RNA editing and the
mitochondrial cryptogenes of kinetoplastid protozoa." Cell. 57, 355-366
(1989)
73. Shaw, J., Campbell, D. and Simpson, L., "Internal
frameshifts within the mitochondrial genes for cytochrome oxidase
subunit II and maxicircle unidentified reading frame 3 of Leishmania
tarentolae are corrected by RNA editing: Evidence for translation of the
edited cytochrome oxidase subunit II mRNA." Proc. Natl. Acad. Sci. USA.
86, 6220-6224 (1989)
74. Simpson, A.M., Suyama, Y., Dewes, H., Campbell, D. and
Simpson, L., "Kinetoplastid mitochondria contain functional tRNAs which
are encoded in nuclear DNA and also contain small minicircle and
maxicircle transcripts of unknown function." Nucl. Acids Res. 17,
5427-5445 (1989) (PDF) 75. Campbell, D., Suyama, Y. and Simpson, L., "Genomic
organization of nuclear tRNAGly and tRNALeu genes in Trypanosoma
brucei". Mol. Biochem. Parasitol. 37, 257-262 (1989) 76. Bakalara, N., Simpson, A.M. and Simpson, L., "The
Leishmania kinetoplast-mitochondrion contains terminal
uridylyltransferase and RNA ligase activities" J. Biol. Chem. 264,
18679-18686 (1989)
77. Blum, B., Bakalara, N. and Simpson, L., "A model for
RNA editing in kinetoplastid mitochondria: "Guide RNA" molecules
transcribed from maxicircle DNA provide the edited information." Cell.
60, 189-198 (1990)
78. Sturm, N. and Simpson, L., "Partially edited mRNAs for
cytochrome b and subunit III of cytochrome oxidase from Leishmania
tarentolae mitochondria: RNA editing intermediates." Cell 61, 871-878
(1990) 79. Sturm, N. and Simpson, L., "Kinetoplast DNA
minicircles encode guide RNAs for editing of cytochrome oxidase subunit
III mRNA." Cell. 61, 879-884 (1990)
80. Blum, B. and Simpson, L., "Guide RNAs in kinetoplastid
mitochondria have a non-encoded 3' oligo(U) tail involved in recognition
of the preedited region. Cell 62, 391-397 (1990)
81. Avila, H., Goncalves, A., Nehme, N., Morel, C. and
Simpson, L., "Schizodeme analysis of Trypanosoma cruzi stocks from South
and Central America by analysis of PCR-amplified minicircle variable
region sequences." Mol. Biochem. Parasitol. 42, 175-188 (1990)
82. Simpson, L. "RNA editing--A Novel Genetic
Phenomenon?". Science 250, 512-513 (1990)
83. Blum, B., Sturm, N., Simpson, A.M. and Simpson, L.
"Chimeric gRNA/mRNA molecules with oligo- (U) tails covalently attached
at sites of RNA editing suggest that uridine addition occurs by
transesterification." Cell 65, 1-8 (1991)
84. Degrave, W., Thiemann, O., Goncalves, A., Avila, H.,
Sturm, N., Simpson, L. and Morel, C.M. "Development of tools for
diagnosis and epidemiological studies of Chagas Disease: Detection and
strain typing." In Molecular and Immunological Aspects of Parasitism
(Wang, C.C., Ed.) Amer. Assoc. Advance. Sci. pp. 177-185
(1991) 85. Lake, J., , de la Cruz, V., Ferreira, P., Morel, C.
and Simpson, L., "Evolution of parasitism: Kinetoplastid protozoan
history reconstructed from mitochondrial rRNA sequences (Correction)"
Proc. Natl. Acad. Sci. U.S.A. 88, 2612 (1991)
86. Avila,H.A., Sigman,D.S., Cohen,L.M., Millikan,R.C. and
Simpson, L., "Polymerase chain reaction amplification of Trypanosoma
cruzi kinetoplast minicircle DNA isolated from whole blood lysates:
diagnosis of chronic Chagas' disease." Mol. Biochem. Parasitol. 48,
211-222 (1991) 87. Sturm,N.R. and Simpson, L., "Leishmania tarentolae
minicircles of different sequence classes encode single guide RNAs
located within the variable region approximately 150 bp from the
conserved region." Nucl. Acids Res. 19, 6277-6281 (1991)
88. Maslov, D.A., Sturm, N.R., Niner, B.M.,
Gruszynski,E.S., Peris, M. and Simpson, L., "An intergenic G-rich region
in Leishmania tarentolae kinetoplast maxicircle DNA is a pan-edited
cryptogene encoding ribosomal protein S12." Mol. Cell. Biol. 12, 56-67
(1992)
89. Rovai, L., Tripp, C., Stuart, K. and Simpson, L.,
"Recurrent polymorphisms in small chromosomes of Leishmania tarentolae
after nutrient stress or subcloning." Mol. Biochem. Parasitol. 50,
115-126 (1992) 90. Simpson, A.M., Bakalara, N. and Simpson, L., "A
ribonuclease activity is activated by heparin or by digestion with
proteinase K in mitochondrial extracts of Leishmania tarentolae." J.
Biol. Chem. 267, 6782-6788 (1992)
91. von Haeseler, A., Blum, B., Simpson, L., Sturm, N. and
Waterman, M.S., "Computer methods for locating kinetoplastid
cryptogenes." Nucl. Acids Res. 20, 2717-2724 (1992) 92. Maslov, D.A. and Simpson, L., "The polarity of editing
within a multiple gRNA-mediated domain is due to formation of anchors
for upstream gRNAs by downstream editing." Cell 70, 459-467 (1992)
93. Sturm, N.R., Maslov, D.A., Blum, B. and Simpson, L.
"Generation of unexpected editing patterns in Leishmania tarentolae
mitochondrial mRNAs: Misediting produced by misguiding." Cell 70,
469-476 (1992) 94. Blum, B. and Simpson, L., "Formation of gRNA/mRNA
chimeric molecules in vitro, the initial step of RNA editing, is
dependent on an anchor sequence." Proc. Natl. Acad. Sci. USA, (1992) 89,
11944-11948. 95. Simpson, L., Maslov, D.A. and Blum, B., "RNA editing
in Leishmania mitochondria." In RNA Editing: The Alteration of Protein
Coding Sequences of RNA. (Benne, R., Ed.) Ellis Horwood Series in
Molecular Biology, Simon and Schuster International Group, Hemel
Hempstead, 53-83 (1993) 96. Avila, H.A., Borges Pereira, J., Thiemann, O.,
DePaiva, E., Degrave, W., Morel, C., and Simpson, L., "Detection of
Trypanosoma cruzi in blood specimens of chronic chagasic patients by
polymerase chain reaction amplification of kinetoplast minicircle DNA:
comparison with serology and xenodiagnosis." J. Clin. Microbiol. 31,
2421-2426 (1993)
97. Stuart K., Tripp, C., Maslov, D., Simpson, L. and
Myler, P.J., "The molecular and transcriptional organization of the
multicopy circular form of the LD1 genomic sequence of Leishmania.",
Proceedings of the CSIR Golden Jubilee Symposia, in Current Trends In
Leishmania Res., A.N. Bhaduri, et al, eds., Publications &
Information Directorate (CSIR), New Delhi, 137-151 (1993) 98. Maslov, D.A., Elgort, M.G., Wong, S., Peckova, H. Lom,
J., Simpson, L. and Campbell, D.A., "Organization of mini-exon and
5srRNA genes in the kinetoplastid Trypanoplasma borelli." Mol. Biochem.
Parasitol. 61, 127-136 (1993)
99. Scheinman, A., Aguinaldo, A., Simpson, A., Peris, M.,
Simpson, L. and Lake, J., Reconstitution of a minimal small ribosomal
subunit." In The Translation Apparatus, (K. Nierhaus, S. Franceschi, A.
Subramanian, E. Erdmann and B. Wittmann-Liebold, Eds.) Plenum Press, New
York, pp.719-726 (1993)
100. Simpson, L., Simpson, A.M., and Blum, B., "RNA
editing in Leishmania mitochondria". In RNA Processing - A Practical
Approach. (Hames D. and Higgins, S., Eds.) IRL Press, (1993)
101. Maslov, D.A., Avila, H.A., Lake, J. A., and Simpson,
L., "Evolution of RNA Editing in Kinetoplastid Protozoa: Pan Editing
Preceded 5'-Editing." Nature 368, 345-348, (1994)
102. Peris, M., Frech, G.C., Simpson, A.M., Bringaud, F.,
Byrne, E., Bakker, A., and Simpson, L. "Characterization of two classes
of ribonucleoprotein complexes possibly involved in RNA editing from
Leishmania tarentolae mitochondria." EMBO Journal 13, 1664-1672 (1994) 103. Simpson, L., and Maslov, D.A. "RNA Editing and the
Evolution of Parasites." Science 264, 1870-1871 (1994)
104. Simpson, L. and Maslov, D.A. "Ancient origin of RNA
Editing in kinetoplastid Protozoa". In Current Opinion in Genetics and
Development 4, 887-894 (1994)
105. Maslov, D.A. and Simpson, L. "Evolution of Parasitism
in Kinetoplastid Protozoa." Parasitology Today 11, 30-32 (1994)
106. Maslov, D.A. and Simpson, L. "RNA editing and
mitochondrial genomic organization in the Cryptobiid kinetoplastid
protozoan Trypanoplasma borreli." Mol. Cell. Biol. 14, 8174-8182 (1994)
107. Thiemann, O.H., Maslov, D.A. and SImpson, L.
"Disruption of RNA editing in Leishmania tarentolae by the loss of
minicircle-encoded guide RNA genes." EMBO J. 13, 5689-5700 (1994)
108. Maslov, D.A., Thiemann, O. and Simpson, L. "Editing
and misediting of transcripts of the kinetoplast maxicircle G5
cryptogene in an old laboratory strain of Leishmania tarentolae." Mol.
Biochem. Parasitol. 68, 155-159 (1994)
109. Simpson, L. "From Molecular Evolution to Biomedical
Research: The Case of Charles Darwin and Chagas' Disease". In Evolution
and the Molecular Revolution (Marshall, C. and Schopf, J.W., eds.)
Boston: Jones and Bartlett. 125-148 (1995) (PDF) 110. Frech, G. C., Bakalara, N., Simpson, L. and Simpson,
A. "In vitro RNA Editing-like Activity in a Mitochondrial Extract from
Leishmania tarentolae". EMBO Journal. 14, 178-187 (1995)
111. Bringaud, F., Peris, M., Zen, K.H., and Simpson, L.
"Characterization of two nuclear-encoded protein components of
mitochondrial ribonucleoprotein complexes from Leishmania tarentolae".
Mol. Biochem. Parasitol. 71, 65-79 (1995)
112. Byrne, E., Bringaud, F., and Simpson, L. "RNA-protein
interactions in the ribonucleoprotein T-complexes in a mitochondrial
extract from Leishmania tarentolae". Mol. Biochem. Parasitol. 72, 65-76
(1995)
113. Simpson, L. and Thiemann, O. "Sense from Nonsense:
RNA Editing in Mitochondria of Kinetoplastid Protozoa and Slime Molds".
Cell. 81, 837-840, (1995)
114. Yasuhira, S. and Simpson. L. "Minicircle-encoded
guide RNAs from Crithidia fasciculata." RNA. 1, 634-643 (1995) 115. Bringaud, F., Peyruchaud, S., Baltz, D., Giroud, C.,
Simpson, L. and Baltz, T. "Molecular characterization of the
mitochondrial heat shock protein 60 gene from Trypanosoma brucei". Mol.
Biochem. Parasitol. 74, 119-123 (1995)
116. Avila, H.A., and Simpson, L., "Organization and
Complexity of Minicircle-encoded guide RNAs in Trypanosoma cruzi. RNA.
1, 939-947 (1995)
(PDF) 1996 117. Simpson, L. and Emeson, R. "RNA Editing". Annu. Rev. Neurosci. 19, 27-52 (1996) 118. L. Simpson, G. C. Frech, and D. Maslov. "RNA Editing in Trypanosomatid Mitochondria". In Methods In Enzymology (G. Attardi, ed.) 264, 99-121 (1996) 119. Maslov, D.A., Lukes, J., Jirku, M. and Simpson, L., "Phylogeny of Trypansomes as inferred from the small and large subunit rRNAs: implications for the evolution of parasitism in the trypanosomatid protozoa". Mol. Biochem. Parasitol. 75. 197-206 (1996) 120. Ramos, A., Maslov, D.A., Fernandes, O., Campbell, D.A. and Simpson, L., "Detection and Identification of Human Pathogenic Leishmania and Trypanosoma Species by Hybridization of PCR-amplified Mini-Exon Repeats". Exptl. Parasitol. 82, 242-250 (1996) 121. Lima, B. and Simpson, L., "Sequence-dependent in vivo importation of tRNAs into the mitochondrion of Leishmania tarentolae". RNA 2, 429-440 (1996) 122. Frech, G.C. and Simpson, L. " Uridine Insertion into Pre-edited mRNA by a Mitochondrial Extract from Leishmania tarentolae: Stereochemical Evidence for the Enzyme Cascade Model." Mol. Cell. Biol. 16, 4584-4589 (1996) 123. Thiemann, O.H. and Simpson, L. "Analysis of the 3' uridylylation sites of guide RNAs from Leishmania tarentolae". Mol. Biochem. Parasitol. 79, 229-234 (1996) 124. Yasuhira, S. and Simpson, L. "Guide RNAs and guide RNA genes in the cryptobiid kinetoplastid protozoan, Trypanoplasma borreli." RNA. 2, 1153-1160 (1996) 125. Byrne, E., Connell, G.J., and Simpson, L. "Guide RNA-directed uridine-insertion RNA editing in vitro." EMBO Journal. 15, 6758-6765 (1996) 125a. Lyubchenko Y.L., Blankenship R.E., Gall A.A., Lindsay S.M., Thiemann O., Simpson L., Shlyakhtenko L.S. "Atomic force microscopy of DNA, nucleoproteins and cellular complexes: the use of functionalized substrates." Scanning Microsc Suppl;10:97-107; discussion 107-9 (1996) 1997 126. Connell, G.J., Byrne, E., and Simpson, L. "Guide RNA-independent and Guide RNA-dependent Uridine-Insertion into Cytochrome b mRNA in a Mitochondrial Lysate from Leishmania tarentolae." J. Biol. Chem. 14, 4212-4218 (1997) 127. Yasuhira, S. and Simpson, L. "Phylogenetic affinity of mitochondria of Euglena gracilis and kinetoplastids using cytochrome oxidase I and hsp60. J. Mol. Evol. 44, 341-347 (1997) 128. Peris, M., Simpson, A.M., Grunstein, J., Liliental, J.E., Frech, G.C. and Simpson, L. "Native gel analysis of ribonucleoprotein complexes from a Leishmania tarentolae mitochondrial extract." Mol. Biochem. Parasitol. 85, 9-24 (1997) 129. Simpson, L. "The genomic organization of guide RNA genes in kinetoplastid protozoa: several conundrums and their solutions." Mol. Biochem. Parasitol. 86, 133-141(1997) 130. Bringaud, F, Stripecke, R., Frech, G.C., Freedland, S., Turck, C., Byrne, E. and Simpson, L. "Mitochondrial Glutamate Dehydrogenase from Leishmania tarentolae is a Guide RNA-binding Protein." Mol. Cell Biol. 17, 3915-3923 (1997) 131. Simpson, L. and Thiemann, O.H., "mRNA editing". In Eukaryotic Messenger RNA Processing (Krainer, A., Ed.) Frontiers in Molecular Biology, IRL Press, London, 335-376 (1997) 132. Simpson, L. and Kretzer, F. "The mitochondrion in dividing Leishmania tarentolae cells is symmetric and circular and becomes a single asymmetric tubule in non-dividing cells due to division of the kinetoplast portion." Mol. Biochem. Parasitol. 87, 71-78 (1997) 133. Alfonzo, J.D., Thiemann, O. and Simpson, L. "The mechanism of U-insertion/deletion RNA editing in kinetoplastid mitochondria." Nucl. Acids Res. 25, 3751-3759 (1997) (cover) 1998 134. Simpson, L., Wang, S., Thiemann, O.H., Alfonzo, J.D., Maslov, D. and Avila, H. "U-insertion/deletion Edited Sequence Database." Nucl. Acids Res. 26, 170-176 (1998) 135. Simpson, L. "A base called J." Proc. Natl. Acad. Sci. USA. (Commentary) 95, 2037-2038 (1998) 136. Aphasizhev, R., Karmarkar, U. and Simpson, L. "Are tRNAs imported into the mitochodria of kinetoplastid protozoa as 5'-extended precursors?". Mol. Biochem. Parasitol. 15, 73-80 (1998) 137. Thiemann, O.H., Alfonzo, J.D. and Simpson, L. "Cloning and characterization of Leishmania tarentolae adenine phosphoribosyltransferase." Mol. Biochem. Parasitol. 95, 141-146 (1998) 138. Alfonzo, J.D., Thiemann, O.H. and Simpson, L. "Purification and characterization of MAR1: a mitochondrial associated ribonuclease from Leishmania tarentolae. J. Biol. Chem. 273, 30003-30011 (1998) 139. Connell, G.J. and Simpson, L. "The Role of RNA Structure in RNA Editing". In RNA Structure and Function (R. Simons and M. Grunberg-Manago, eds.), Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y. (1998) 140. Simpson, L. "RNA Editing: An Evolutionary Perspective." In RNA World, Second Edition (R. Gesteland, J. Atkins and T. Cech, eds.) Cold Spring Harbor Press. Cold Spring Harbor, N.Y. (1998) 141. Simpson, L. "RNA Editing" In Frontiere Della Vita (G. Attardi and F. Amaldi, eds.) Instituto della Encliclopedia Italiana, Fondata da Giovanni Trecanni. Origin and Evolution of Life, Vol. I, 483-496 (1998) 1999 142. Kapushoc, S. and Simpson, L. "In vitro uridine insertion RNA editing mediated by cis-acting guide RNAs." RNA. 5, 656-669 (1999) (PDF) 143. Estevez, A.M., Kierszenbaum, F., Wirtz, E., Bringaud, F. Grunstein, J. and Simpson, L. "Knockout of the glutamate dehydrogenase gene in bloodstream Trypanosoma brucei in culture has no effect on editing of mitochondrial mRNAs." Mol. Biochem. Parasitol. 100, 5-17 (1999) 144. Maslov, D.A., Yasuhira, S. and Simpson, L. "Phylogenetic affinities of Diplonema within the Euglenozoa as inferred from the SSU rRNA gene and partial COI protein sequence. Protist 150, 33-42 (1999) 145. Simpson, L. and Maslov, D.A. "Evolution of the U-insertion/deletion RNA editing in mitochondria of kinetoplastid protozoa." In Molecular Strategies in Biological Evolution (L.H. Caporale, ed.) Annal. N.Y. Acad. Sci. 870, 190-205 (1999) 146. Simpson, L. "Protists on the Web." Protist. 150, 109-112 (1999) (On-line text) 147. Blanc, V., Alfonzo, J.D., Aphasizhev, R. and Simpson, L. "The Mitochondrial RNA Ligase from Leishmania tarentolae Can Join RNA Molecules Bridged by a Complementary RNA." J. Biol. Chem. 274, 24289-24296 (1999) 148. Estevez, A.E., Thiemann, O.H. Alfonzo, J.D. and Simpson, L. "T7 RNA polymerase-driven transcription in mitochondria of Leishmania tarentolae and Trypanosoma brucei." Mol. Biochem. Parasitol. 103, 251-259 (1999) 149. Alfonzo, J.D., Blanc, V., Estevez, A.M., Rubio, M.A.T. and Simpson, L. "C to U editing of anticodon of imported mitochondrial tRNATrp allows decoding of UGA stop codon in Leishmania tarentolae." EMBO J. 18, 7056-7062 (1999) 150. Estevez, A.M. and Simpson, L., "Uridine insertion/deletion RNA editing in trypanosome mitochondria - a review." Gene. 240, 247-260 (1999) 2000 151. Simpson, L., Thiemann, O.H., Savill, N.J., Alfonzo, J.D. and Maslov, D.A. "Evolution of RNA Editing in Trypanosome Mitochondria". Proc. Natl. Acad. Sci. USA. 97, 6986-6993 (2000) (PDF) 152. Rubio, M.A., Liu, X., Yuzawa, H., Alfonzo, J.D. and Simpson, L. "Selective Importation of RNA into Isolated Mitochondria from Leishmania tarentolae." RNA. 6, 988-1003 (2000) 153. Stephen T. Kapushoc, Juan D. Alfonzo, Mary Anne T. Rubio, and Larry Simpson. "End processing precedes mitochondrial importation and editing of tRNAs in Leishmania tarentolae". J. Biol. Chem. 275, 37907-14 (2000) 2001154. Carillo, R., Thiemann, O.H., Alfonzo, J.D. and Simpson, L. (2001) Uridine insertion/deletion RNA editing in Leishmania tarentolae mitochondria shows cell cycle dependence. Mol. Biochem. Parasitol. 113 175-181 155. Aphasizhev, R. and Simpson, L. (2001) Isolation and characterization of a U-specific 3'-5' exonuclease from mitochondria of Leishmania tarentolae. J. Biol. Chem. 276, 21280-21284 156. Simpson, L. (2001) Protist Web Alert: From Parasites to Life in Space! Protist. 152(1), 31-2 157. Simpson, L. (2001) Protist Web Alert: Some Marine Protists of Ill-Repute. Protist 152, 113-114 (PDF) 158. Simpson, L. (2001) RNA Editing. In Frontiers of Science, Academic Press. Vol. 1: The Origins of Life (PDF) 159. Gao, G., Kapushoc, S., Simpson, A.M., Thiemann, O.H. and Simpson, L. Guide RNAs of the recently isolated LEM125 strain of Leishmania tarentolae: an unexpected complexity. RNA. 7(9),1335-47. 160. Simpson, L. (2001) Protist Web Alert: Memories of Times Past. Protist 152, 183-184 (PDF) 161. Simpson, L. (2001) Protist Web Alert: The Chlamy Crowd on the Web. (PDF) 162. Simpson, L. (2001) Meeting Report: World Leish II, Crete, Greece, May 20-24, 2001. Protist 152,169-173 (PDF) 2002163. Kapushoc, S. T., Alfonzo, J. A. and Simpson, L. Differential localization of nuclear-encoded tRNAs between the cytosol and mitochondrion in Leishmania tarentolae. RNA. 8, 57-68 (2002) (PDF) 164. Ruslan Aphasizhev, Sandro Sbicego, Marian Peris, Sei-Heon Jang, Inna Aphasizheva, Agda M. Simpson, Anatoly Rivlin and Larry Simpson. Trypanosome Mitochondrial 3' Terminal Uridylyl Transferase (TUTase): The Key Enzyme in U-insertion/deletion RNA Editing. Cell 108, 637-648 (2002) 165. Simpson, L. Protist Web Alert: Malaria- The Biological Terminator. Protist 153, 7-8 (2002) 166. Simpson, L. (2002) Protist Web Alert: Poetry and Pictures. Protist 153, 109-110 (2002) 167. Crain, P.F., Alfonzo, J.D., Rozenski, J., Kapushoc, S.T., McCloskey, J.A. and Simpson, L. (2002) Modification of the universally unmodified uridine-33 in a mitochondria-imported edited tRNA and the role of the anticodon arm structure on editing efficiency. RNA. 8, 752-761 168. Monzani, P.S., J. D. Alfonzo, L. Simpson, G. Oliva and O.H. Thiemann (2002) Cloning, characterization and preliminary crystallographic analysis of Leishmania hypoxanthine-guanine phosphoribosyl transferase. Biochim. Biophys. Acta 1598, 3-9 169. Simpson, L. (2002) Protist Web Alert: The Ciliates Among Us. Protist. 153, 221-222. (PDF) 174. Simpson, L. (2002) Protist Web Alert: The Best of Times. Protist. 153, 365. 2003171. Aphasizhev, R., Aphasizheva, I., Nelson R. and Simpson, L. (2003) A 100 kDa complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components. RNA. 9, 62-76 172. Tomonori Kaneko, Takeo Suzuki, Stephan T. Kapushoc, Mary Anne Rubio, Jafar Ghazvini, Kimitsuna Watanabe, Larry Simpson and Tsutomu Suzuki. (2003)Wobble modification differences and subcellular localization of tRNAs in Leishmania tarentolae: implication for tRNA sorting mechanism. EMBO J. 22, 657-667 173.Ruslan Aphasizhev, Inna Aphasizheva, Robert E. Nelson, Guanghan Gao, Agda M. Simpson, Xuedong Kang, Arnold M. Falick, Sandro Sbicego and Larry Simpson. (2003) Isolation of a U-insertion/deletion editing complex from Leishmania tarentolae mitochondria. EMBO J. 22, 913-934. (PDF) (Supplementary material) (Sequences not yet in Genbank) 174. Simpson, L., Sbicego, S. and Aphasizhev, R. (2003) Review: Uridine insertion/deletion RNA editing in trypanosome mitochondria: A complex business. RNA. 9, 265-276 175. Sandro Sbicego, Juan D. Alfonzo, Antonio M. Estévez, Mary Anne T. Rubio, Xuedong Kang, Christoph W. Turck , Marian Peris and Larry Simpson (2003) RBP38, a novel RNA-binding protein from trypanosomatid mitochondria, modulates RNA stability. Eukary. Cell. 2, 560-568. 176. Hong, M. and Simpson, L. Genomic Organization of Trypanosoma brucei Kinetoplast DNA minicircles. Protist. (2003) 154, 265-279 177. Gao, G. and Simpson, L. Is the Trypanosoma brucei REL1 RNA ligase specific for U-deletion editing and the REL2 RNA ligase specific for U-insertion editing? J. Biol. Chem. 278(30):27570-4 178. Aphasizhev, R., I. Aphasizheva and L. Simpson. (2003) A tale of two Tutases. Proc. Natl. Acad. Sci. USA. 100: 10617-10622 (PDF) (Commentary by Jonatha Gott) 2004179. Kang, X., Falick, A.M., Nelson, R.E., Gao, G., Rogers, K., Aphasizhev, R. and Simpson, L. (2004) Disruption of the zinc finger motifs in the Leishmania tarentolae LC-4 (=TbMP63) L-complex editing protein affects the stability of the L-complex. J. Biol. Chem. 279(6):3893-9 180. Martina Neboháová, Dmitri A. Maslov, Arnold M. Falick and Larry Simpson (2004) The Effect of RNAi Down Regulation of RET1 3' TUTase on Mitochondrial de novo Protein Synthesis and Stability of Respiratory Complexes in Trypanosoma brucei. J. Biol. Chem. 279: 7819-7825 181. Larry Simpson, Ruslan Aphasizhev, Guanghan Gao and Xuedong Kang Simpson, L. (2004) Mitochondrial proteins and complexes in Leishmania and Trypanosoma involved in U-insertion/deletion RNA editing. RNA. 10: 159-170 182. Robert E. Nelson, Inna Aphasizheva, Arnold M. Falick, Martina Nebohacova and Larry Simpson (2004) The I-complex in Leishmania tarentolae is a uniquely-structured F1-ATPase. Mol.Biochem.Parasitol. 135, 221-224 183. Inna Aphasizheva, Ruslan Aphasizhev and Larry Simpson (2004) RNA Editing Terminal Uridylyl Transferase 1: Identification of Functional Domains by Mutational Analysis. J. Biol. Chem. 279, 24123-24130 184. Simpson, L. (2004) A Shameless Advertisement. Protist. 155 185. Aphasizhev, R., Aphasizheva, I. and Simpson, L. (2004) Multiple Terminal Uridylyltransferases of Trypanosomes. FEBS Letters. 572, 15-28 2005186. Kang, Xuedong, Rogers, Kestrel, Gao, Guanghan, Falick, Arnold M., Zhou, Sharleen and Simpson, Larry (2005) Reconstitution of uridine deletion pre-cleaved RNA editing with two recombinant enzymes. Proc. Natl. Acad. Sci. USA. 102: 1017-1022 187. Guanghan Gao, Agda M. Simpson, Xuedong Kang, Kestrel Rogers, Martina Nebohacova and Larry Simpson (2005) Functional complementation of Trypanosoma brucei RNA in vitro editing with recombinant RNA ligase. Proc. Natl. Acad. Sci. USA. 102, 4712-4717 188. Trager, L., Spielman, A. and Simpson L. (2005) Malaria, Not Down and Certainly Not Out. A,. J. Trop. Med. Hyg. 73, 824-5 189. Sherman, I. and Simpson L. (2005) In Memoriam: William Trager (1910-2005) J Eukaryot Microbiol. 52(3):173-6
190. Simpson, L. (2005) Uridine insertion/deletion RNA editing as a paradigm for site-specific modifications of RNA molecules. (Gesteland, Cech and Atkins, eds.) The RNA World, Third Edition. Cold Spring Harbor Press, N.Y. 2005 (PDF) 191. Simpson, L. (2006) Who am I? Protist. 157, 89 192. Simpson, L. (2006) Protists on the Web - Redoux. Protist. 157, 255-258 193. Maslov, D., Butler, E., Sharma, M., Falick, A., Gingery, M., Agrawal, R., Spremulli, L. and Simpson, L. (2006) Isolation and Characterization of Mitochondrial Ribosomes and Ribosome Subunits from Leishmania tarentolae. Mol. Biochem. Parasitol. 148, 69-78 194. Kang, X., Gao, G., Rogers, K., Falick, A., Zhou, S. and Simpson, L. (2006) Reconstitution of full round uridine-deletion RNA editing with three recombinant proteins. Proc. Natl. Acad. Sci. USA. 103, 13944-13949 (PDF) 194. Simpson, L. (2006) Death of the "ologies". Protist 157, 361-362 2007 195. Simpson, L. (2007) My Personal Epiphany. Protist. 158 (PDF) 196. Maslov, D., Spremulli, L. Sharma, M., Bhargava, K., Grasso, D., Falick, A., Agrawal, R., Parker, C. and Simpson, L. (2007) Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae. Mol. Biochem. Parasitol. 152(2):203-12 (PDF) 197. Maslov, D. and Simpson, L. (2007) Strategies of Cryptogene Discovery and Analysis. Methods in Enzymol. 2007;424:127-39 (PDF) 198. Rogers, K, Gao, G. and Simpson, L. (2007) Uridylate-Specific 3'-5' exoribonucleases involved in Uridylate-deletion RNA editing in trypanosomatid mitochondria. J. Biol. Chem. 282:29073 (PDF) 2008 199. Simpson, L. (2008) What is Science and why do we do it? Protist 159, 3 (PDF) 200. Jensen, R.E., Simpson, L. and Englund, P. (2008) What happens when Trypanosoma brucei leaves Africa? Trends in Parasitol. 24: 428-431 (PDF 2009 201. Osato, D., Rogers, K., Li, Feng, Guo, Qiang, Richmond, Greg, Klug, Felix and Simpson, L.. In search of the editosome. (2009) RNA. 15:1338-1344 (Suppl. material) 202. Simpson, L. Science is a Hard Mistress. (2009) Protist. 160: 481-482 203. Manjuli R. Sharma, Timothy M. Booth, Larry Simpson, Dmitri A. Maslov, and Rajendra K. Agrawal. Structure of a mitochondrial ribosome with minimum RNA. (2009) PNAS. 106: 9637-9642 21. 204. Li, F., Ge, P., Hui, W. Atanasov, I, Rogers, K., Guo, Q., Osato, D., Falick, A., Zhou, H. and Simpson, L. Structure of the core editing complex (L-complex) involved in uridine insertion/deletion RNA editing in trypanosomatid mitochondria. (2009) PNAS 106:12306-12310. (PDF) (PDF of Supplemental Material) (AVI movie of single particle reconstruction) 205. Simpson, L. (2009). Musings on the real problems. Protist. (2009) 160(1): 3 206. Simpson, L., Aphasizhev, R., Lukes, J. and Cruz-Reyes, J. (2009). Guide to the nomenclature of kinetoplastid editing. Protist. 161, 2-6 207. Nebohácová M, Kim CE, Simpson L, Maslov DA. (2009) RNA editing and mitochondrial activity in promastigotes and amastigotes of Leishmania donovani. Int J Parasitol. 2009 39(6):635-44 2010 208. Gao, G., Rogers, K., Li, F., Guo, Q., Osato, D., Zhou, S., Falick, A. and Simpson, L. (2010). Uridine insertion/deletion RNA editing in Trypanosomatids: Specific stimulation in vitro of Leishmania tarentolae REL1 RNA ligase activity by the MP63 zinc finger protein. Protist. 161, 489-496 209. Simpson, L. (2010). A Personal Scientific Odyssey. (2011) Protist 162, 188-206 (PDF) 2011 210. Feng Li, Jeremy Herrera, Sharleen Zhou and Larry
Simpson. (2011) 2012 211. Eckers, E., Cyrklaff, C., Simpson, L. and Deponte, M. (2012). Mitochondrial protein import pathways are functionally conserved among eukaryotes despite compositional diversity of the import machineries. J. Biol. Chem. 393, 513-524 (Suppl. Material) (Cover) 212. Simpson, L. (2012) The Wonder of it All. Trends in Genetics. 28(9): 419-420. 2013 213. Sherman, I. and Simpson L. (2013). William Trager - 1910-2005. A Biographical Memoir. Proc. Natl. Acad. Sci US 2015 214. Simpson,L.(2015). The most unlikely places. RNA 21:740215. Simpson, Douglass, Lake, Pelligrini and Li (2015). PLOS Neglected Diseases. Comparison of the Mitochondrial Genomes and Steady State Transcriptomes of Two Strains of the Trypanosomatid Parasite, Leishmania tarentolae (PLOS web site) 216. Wong, R.G., Kazane, K., Maslov, D., Rogers, K., Aphasizhev, R. and Simpson. L. (2015).U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA. Mitochondrion. (PDF) (OnLine version) 2019217. Klatt S., Simpson L., Maslov, D. and Konthur, Z. (2019). Leishmania tarentolae: Taxonomic classification and its application as a promising biotechnological expression host. PLOS Negl Trop Diseases 13: In Press2020218. Aphasizheva, I., Alfonzo, J., Carnes, J., Cestari, I., Cruz-Reyes, J., Goringer, U., Hajduk, D., Lukes, J., Madison-Antenucci, S., Maslov, D., McDermont, S., Ochenreiter, T., Read, L., Salavati, R., Schnaufer, A., Simpson, L., Stuart, K., Yurchenko, V., Zhou, Z., Zikova, A., Zhang, L., Zimmer, S. and Aphasizhev, R. (2020). Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes. Trends in Parasitol. In Press. |