PPT Slide
Crude indicates the 12,000 x g - 20 min clarified supernatant of the CHAPS lysate (as described in
Experimental Procedures). S-100 is the supernatant after centrifugation of the crude fraction at 100,000 x g for 1 hr. Mono Q, Sup-12 and Mono P represent the pooled peaks of nuclease activity from each chromatography step.
A unit (U) is defined as 1 nmol of substrate RNA cleaved per min.