LOCUS KTKPMCD 966 bp DNA circular ORG 14-NOV-2002
DEFINITION T.brucei kinetoplastid minicircle H40 DNA, complete.
ACCESSION M15324 M15303
KEYWORDS minicircle.
SOURCE
ORGANISM Trypanosoma brucei
Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
COMMENT A 13 base pair sequence, within the conserved region of about 130
base pairs (positions 789-920), is conserved among kinetoplastid
genera [1]. Minicircle fragments containing the conserved sequence
and a 5' adjacent segment, starting at position 538 with an 18 base
pair sequence that occurs as 3 or 4 pairs of imperfect inverted
repeats in African trypanosomes [2], exhibit anomalous
electrophoretic mobility in polyacrylomide gels [1]. The position
of the oligo (dA) tracts in the fragments appears to influence this
anomalous ability [1]. The presence of conserved features
independent of conserved nucleotide sequence and the lack of
conserved open reading frames among these minicircles suggests that
mincircles may have a function other than encoding protein [1]. The
18 base pair sequence is polar, and one half is almost perfectly
conserved [2]. The distribution of the 18 base pair sequences in
minicircles defines two classes of sequences: A segments vary
considerably in length and contain about 10% more "g" + "c" than B
segments, which are all about 100 base pairs long. B segments along
with their terminal 18 base pair sequences superficially resemble
insertion sequences [2].
COMMENT EMBLRFLN|RN [1]
COMMENT EMBLRFLN|RP 1-966
COMMENT EMBLRFLN|RX MEDLINE; 86174895.
COMMENT EMBLRFLN|RA Jasmer D.P., Stuart K.D.;
COMMENT EMBLRFLN|RT "Conservation of kinetoplastid minicircle characteristics without
COMMENT EMBLRFLN|RT nucleotide sequence conservation";
COMMENT EMBLRFLN|RL Mol. Biochem. Parasitol. 18:257-269(1986).
COMMENT EMBLRFLN|RN [2]
COMMENT EMBLRFLN|RC 18 bp inverted repeats and flanks
COMMENT EMBLRFLN|RX MEDLINE; 86174900.
COMMENT EMBLRFLN|RA Jasmer D.P., Stuart K.;
COMMENT EMBLRFLN|RT "Sequence organization in African trypanosome minicirclesw is defined by 18
COMMENT EMBLRFLN|RT base pair inverted repeats";
COMMENT EMBLRFLN|RL Mol. Biochem. Parasitol. 18:321-331(1986).
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|EMBL
COMMENT VNTDBDATE|283282895|
COMMENT VNTNAME|KTKPMCD|
COMMENT VNTAUTHORNAME|Larry Simpson|
COMMENT VNTOAUTHORNAME|UNKNOWN|
FEATURES Location/Qualifiers
source 160..159
/db_xref="taxon:5691"
/kinetoplast
/organism="Trypanosoma brucei"
/vntifkey="98"
misc_feature 1..9
/vntifkey="21"
/label=CSB-1
/note="Add by Min Hong"
misc_feature 29..36
/vntifkey="21"
/label=CSB-2
/note="Add by Min Hong"
misc_feature 75..87
/vntifkey="21"
/label=CSB-3
/note="Add by Min Hong"
repeat_region 190..205
/vntifkey="34"
/label=RPa
/note="Add by Min Hong"
repeat_region 365..380
/vntifkey="34"
/label=RPb
/note="Add by Min Hong"
repeat_region 574..589
/vntifkey="34"
/label=RPc
/note="Add by Min Hong"
misc_feature 228..266
/vntifkey="21"
/label=gCO3(750-775)
/note="Add by Min Hong"
misc_feature 390..441
/vntifkey="21"
/label=gA6(771-799)
/note="Add by Min Hong"
repeat_region complement(315..331)
/vntifkey="34"
/label=RP1
/note="add by min hong"
repeat_region complement(486..502)
/vntifkey="34"
/label=RP2
/note="Add by min hong"
repeat_region complement(697..714)
/vntifkey="34"
/label=RP3
/note="Add by min hong"
misc_feature 623..644
/vntifkey="21"
/label=gND9(223-241)
misc_feature 948..115
/vntifkey="21"
/label=CON
BASE COUNT 374 a 76 c 190 g 326 t
ORIGIN
1 tgggcgtgca gatttcacca tacacaaatc acgtgctatt tttggcctat ttttaggtcg
61 gaggtacttc gaaaggggtt ggtgtaatac acacagggtt tttcctgggt ttcccgagtt
121 atttgggact ttctggggat ggtgattcta ctgtaggaaa agcttcatca ggttttccaa
181 ggtgagagaa ataagataat agataagatt atagttaata tatagatagt ataatgatat
241 aattaaacaa acgtatatga caagtagatg gcagtgaaat atagtgatta atttattata
301 tatattgtaa tatttatcta ttatcttatt ttaatcctgg tagtgtctga tggatggatg
361 gaaaaatgat ataatagata tgtaatgata gaattaatgt atgaaattaa tatatactat
421 aactccgata acgaatcaga ttttgacagt gatatgataa ttatatataa tgttattgaa
481 taatctattt attataatat ttctttattt tagacgctga aaatcgcagt aaaatgtagt
541 ttaaaagctc attaatgtga ctaaaagaaa gaaaataaag taatagatag gatgaataat
601 aattaatatg atgattatga tatataatcc attatcagtt gcgagattgt agtataaaga
661 ataattaata gttaaattta atataagact aatatttatt tattataaga tttttaaatt
721 agagaagtac gatagaggta gaataaaggg gtatgagaca aggcatgata taaacctgaa
781 aattgatgag gaggtagtaa agatgtggtg tataaggaga aatagaagtt tgtaattgta
841 gataaataat ataatggtcg aggtacttat ttataattga gtaggagtag taaaaaattc
901 gataaaaatt gagagaattc gggtaaaaat ggcttgaaaa atggcttgaa aaaactcaaa
961 atctta
//