LOCUS KTKPMCC 1058 bp DNA circular ORG 16-NOV-2002 DEFINITION T.brucei kinetoplastid minicircle H39 DNA, complete. ACCESSION M15323 M15302 KEYWORDS minicircle. SOURCE ORGANISM Trypanosoma brucei Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma. COMMENT A 13 base pair sequence, within the conserved region of about 130 base pairs (positions 501-633), is conserved among kinetoplastid genera [1]. Minicircle fragments containing the conserved sequence and a 5' adjacent segment, starting at position 199 with an 18 base pair sequence that occurs as 3 or 4 pairs of imperfect inverted repeats in African trypanosomes [2], exhibit anomalous electrophoretic mobility in polyacrylomide gels [1]. The position of the oligo (dA) tracts in the fragments appears to influence this anomalous ability [1]. The presence of conserved features independent of conserved nucleotide sequence and the lack of conserved open reading frames among these minicircles suggests that mincircles may have a function other than encoding protein [1]. The 18 base pair sequence is polar, and one half is almost perfectly conserved [2]. The distribution of the 18 base pair sequences in minicircles defines two classes of sequences: A segments vary considerably in length and contain about 10% more "g" + "c" than B segments, which are all about 100 base pairs long. B segments along with their terminal 18 base pair sequences superficially resemble insertion sequences [2]. Five 14 base pair long degenerate tandem repeats are located at positions 301-432. COMMENT EMBLRFLN|RN [1] COMMENT EMBLRFLN|RP 1-1058 COMMENT EMBLRFLN|RX MEDLINE; 86174895. COMMENT EMBLRFLN|RA Jasmer D.P., Stuart K.D.; COMMENT EMBLRFLN|RT "Conservation of kinetoplastid minicircle characteristics without COMMENT EMBLRFLN|RT nucleotide sequence conservation"; COMMENT EMBLRFLN|RL Mol. Biochem. Parasitol. 18:257-269(1986). COMMENT EMBLRFLN|RN [2] COMMENT EMBLRFLN|RC 18 bp inverted repeats and flanks COMMENT EMBLRFLN|RX MEDLINE; 86174900. COMMENT EMBLRFLN|RA Jasmer D.P., Stuart K.; COMMENT EMBLRFLN|RT "Sequence organization in African trypanosome minicirclesw is defined by 18 COMMENT EMBLRFLN|RT base pair inverted repeats"; COMMENT EMBLRFLN|RL Mol. Biochem. Parasitol. 18:321-331(1986). COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|EMBL COMMENT VNTDBDATE|283442086| COMMENT VNTNAME|KTKPMCC| COMMENT VNTAUTHORNAME|Larry Simpson| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers source 540..539 /db_xref="taxon:5691" /kinetoplast /organism="Trypanosoma brucei" /vntifkey="98" misc_feature 1..9 /vntifkey="21" /label=CSB-1 /note="Add by Min Hong" misc_feature 28..35 /vntifkey="21" /label=CSB-2 /note="Add by Min Hong" misc_feature 75..87 /vntifkey="21" /label=CSB-3 /note="Add by Min Hong" misc_feature 187..214 /vntifkey="21" /label=gMURF2(938-963) /note="Add by Min Hong" misc_feature 377..404 /vntifkey="21" /label=gMURF2(936-963) /note="Add by min hong" misc_feature 860..905 /vntifkey="21" /label=gMURF2(977-1015) /note="Add by min hong" repeat_region complement(503..520) /vntifkey="34" /label=RP1 /note="Add by Min Hong" repeat_region 607..624 /vntifkey="34" /label=RPa /note="Add by Min Hong" repeat_region complement(738..754) /vntifkey="34" /label=RP2 /note="Add by Min Hong" repeat_region 847..864 /vntifkey="34" /label=RPb /note="Add by Min Hong" misc_feature 1018..90 /vntifkey="21" /label=CON misc_feature 661..688 /vntifkey="21" /label=gCR4(129-152) BASE COUNT 384 a 75 c 225 g 374 t ORIGIN 1 agggcgtgca aaaatacaca tacacaaatc cgtgctattt tggggcattt tttgaggtcc 61 gaggtacttc gaaaggggtt ggtgtaatac acacacggtt tttcaaggga tttctcggtt 121 tatctgggtt ttatgggttt tcggggtctg gaggtttcaa aataaaaaga aaatatatta 181 ataaatagaa gtacgtcata atatatataa ttaatatact gtaaagaggg aatatacacc 241 ctctatataa taagatacct atataattgt aataagtata attatctatc tattatttat 301 ttttattgtt tggagcgggt aattaggtgt ttgatggttg atttaggtgt ttattgtaac 361 tgcgaatctg actaataaag ataagataat agataggagt ataaatgata attgagtgat 421 gagatatata attaaattta tgaggatttt attgtagatg atagtgttat atgatgtaat 481 atatattaat tattttgtat attatttatt attttatttt gttcttggag gtgatgatga 541 agctttgatc tactgttgta attgctcata tctaacttct gggattaatt gaatgcctaa 601 aagagagaag tataataata gatacgagat aaataaatat atacatagaa attaatacat 661 aaatcacaaa cgaataaggg gcagaagagt tgtatgatat attaaatatg atgaataata 721 atgattaaat taatacttat ttattatatt attttaatta gtggggttgt taggaggatt 781 aatttgatgg gataaatggg gtggtaagag tggtaagact gaaaaagtta atggtaattg 841 gtcgtaaaat atggttatag aataaattaa agtgtaaggg tgatgtgaag aaagcgttat 901 ggagatttaa tattggagat ttaatattgg agatttaata ttggagattt aatattggag 961 aaatttaata ttggaggctg tagtaggtag gctatgagtg ttgaacgaaa tcatgaaaaa 1021 aatcggggaa aattgcctcg aaaaaaccga aaatctta //