LOCUS pX 6138 bp DNA circular 4-JUL-1999
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|138551800|
COMMENT VNTDBDATE|163515974|
COMMENT VNTNAME|pX|
COMMENT VNTAUTHORNAME|Tristan|
COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
CDS 5287..6078
/vntifkey="4"
/label=Neo\R
/note="Neomycin resistence gene fused to DHFR (lacks ATG initiation codon)"
splicing_signal 4867..5286
/vntifkey="38"
/label=DHFR\SAS
rep_origin complement(3715..4090)
/vntifkey="33"
/label=E.\coli\ori
CDS 2290..3150
/vntifkey="4"
/label=Amr\R
5'UTR 1286..1369
/vntifkey="52"
/label=DST\RNA\5'\UTR
promoter 4239..4258
/vntifkey="30"
/label=T7\promoter
3'UTR 4287..4414
/vntifkey="50"
/label=3'\UTR\from\13k\RNA
/note="Accession no. X51733"
3'UTR 8..1365
/vntifkey="50"
/label=DHFR\3'\UTR
BASE COUNT 1375 a 1718 c 1672 g 1373 t
ORIGIN
1 actagtgatc ccgcccgcac ccgcgcgcgc tcaggccgcg tgtcgtcgtg ctctttccat
61 tttttttttg tgtgtggtgg tgatttgagc tgctctccgt tgtgtgctgg ggaccctcct
121 tccctcgatc tcctcgtgcg gggtctccga cgcgcagacg cggtgcgtgc gagcgtgcac
181 gtgctgtcgc cgtggctgta gttgcgagcg gagagagaga gagagggagg gagggagggc
241 agagggcaga ggacatgcgg gtgggaacgt gcaccggcct cgtctcacgc agctggagcc
301 cacgaatgcc accaccacac ccttcttccc cccccctccc tcttcttccc acggcggcga
361 cgacgacggg ggctcagctc acgcttctgt agggtattat attaaagcac atgtgcgtgc
421 tgtgcttcgg cttcttgttg gtggtcgttc gctttcaagt ccacgactcc tgccgttgct
481 caccgccggg ctgtcttctt tcgcccctcc ctcggtgcct cttccgccgc cgcacgcgtg
541 cgctgatcac gcgtcttgcg tgatgtgcct gtgtgtatac acaccgtgca cggagagagc
601 gagcgagcga gagagagaga gggcgagagg aagaatggcc ggtgcctcgc gtgggcaagc
661 gtgcgcgagt ttgtcgtgtc gctgcgccgc gtggatgtcg cgcagcgcaa gaccccgtgc
721 gcctgaaacg tgaagggagg tcgagaagcg tgccgcatga ggccttaagg caggaaagtg
781 aaaagagctc gacgcgagtg cagcacgcgc gacatcgaac aggacggaat agacactcag
841 ccccttcccc tttcaaaaac tgaatggacg gacatcgtaa cgcgcttctc tcccctccac
901 tccccctgtc cctgtctcct tgacgtgtgc tgctctcgtg agcgacagat gaagcgaggc
961 gtagaaggaa agggggggga gcgaggcctg cacacacaca cacacacaca cacacacaca
1021 cacacacaca cacacacaca cacacacgaa aagcaagttt gaggcagacg tggtttcaca
1081 cgattcctgt gcaagaagcg tgcctgcgtg acggtgtggc tgtgtatgca tgcgtgaagg
1141 gttaccgcaa cgatgcgcaa tggactcccc cgctttccat ttcgtcacct tccgcctctc
1201 tctctctctc tctcaccatc tacgcgtgca catcatcaac tgtctcttgt cggtgctcac
1261 caccctcaac cacccctcac tttcaaggct tcccgaacgc acacaaaagg cgtgaaaacc
1321 gctcgcgtgt gttgagccgt ccaccgtagc cctccccctg tccccggggg atccactagt
1381 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
1441 tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
1501 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
1561 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
1621 gtgccactgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
1681 cctgaatggc gaatggcgcg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
1741 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
1801 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
1861 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
1921 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
1981 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
2041 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
2101 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
2161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
2221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
2281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
2341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
2401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
2461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
2521 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
2581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
2641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
2701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
2761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
2821 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
2881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
2941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
3001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
3061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
3121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
3181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
3241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
3301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
3361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
3421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
3481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
3541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
3601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
3661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
3721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
3781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
3841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
3901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
3961 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
4021 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
4081 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
4141 tgcagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
4201 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
4261 aattgggtac cgggcccccc ctcgaggtcg acggggtgat ggagagaatg gttgaccatg
4321 aggacgccga tgggcgatgc gccagcgcgt gcttgcgtac ttcaatcgtt tttcatcttg
4381 attcagagct aagtagtagc acaccacagc aagcgtaatc atgtagcatg tatgcaggtg
4441 cacgagtagg cactctctgt ctctgtccgg catctatgat gctctccggc cgcgtgcgcg
4501 cgacgcctct gtgtgagtgc tcgtgtgagt gtgtgagagc tgtccaggcc tttcctctgc
4561 ctactcgtcc cacgctcagg caggcacaga ggaatgaaaa ggaagacgag gacgcaacgc
4621 caggcgcatc gacatcgcgc acgtgtttgc ggtgtactgt gcgtcgatga tcgagcggcc
4681 aagcgtccag cttcacgcga ggaggactgc atcgctgctc ctgtgcgcgt gcgtgcacgc
4741 gtcaaggcac gctcgattcg ttcccagggt ggataccctc ccccctcccc cagctgcaga
4801 caacccccac cctctcgagg cttgcaccca ggctcgtcag tctctctacc actttctgcc
4861 ttctgtagtg caccccaccc ctgcattctc ctctctctca cacacacaca cacacacaca
4921 cacacacaca cacacacacg tacacacgta cacacacacg tgcgcatgct tgacgcatac
4981 gcgacgaatt cgaaagctca cctcattcct ccctcctcac accatcatcg gcatccatag
5041 agacacgtgc gcgtagaacg atacagctca cgcgcacaga gaggcgtgtt ctggtcgtgc
5101 gcatcatcgg cacagcactg gcggacgaaa ctcgcacaca ggcacgccgc ctcctttcac
5161 ccgtcataga tagttgaatt agacgccctc ctcctccctc atcatcgccg tcgtcatccg
5221 ggtccgagca ctagtcgcgt gtggcctcga acaccgagcg accctgcagc caatatggga
5281 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
5341 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
5401 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
5461 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
5521 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
5581 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
5641 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
5701 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
5761 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
5821 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
5881 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
5941 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
6001 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
6061 cttgacgagt tcttctgagg ggatcggcaa taaaaagaca gaataaaacg cacgggtgtt
6121 gggtcgtttg ttcggatc
//