LOCUS pLEW90 6190 bp DNA circular 8-JUN-2000 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|138454045| COMMENT VNTDBDATE|197046824| COMMENT VNTNAME|pLEW90| COMMENT VNTAUTHORNAME|Tristan| COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu| FEATURES Location/Qualifiers rep_origin complement(5760..6135) /vntifkey="33" /label=E.\coli\ori /note="E. coli pMB2 replication origin" CDS 1..905 /vntifkey="4" /label=B-tublulin /note="3' region of B-tubuline gene. Accession k02836 Linearizing at NotI site will intergrate plasmid in the B-tubulin locus." 5'UTR 908..1144 /vntifkey="52" /label=Ald\SAS /note="Aldolase splicing acceptor site. Accessioin X52586" misc_feature 1152..1776 /vntifkey="21" /label=Tet\repressor /note="E. coli Tn10 tetR repressor Accession X00694" 3'UTR 1782..1921 /vntifkey="50" /label=Ald\3'\utr /note="Aldolase 3' untranslated region. Accession x52586" 5'UTR 1933..2042 /vntifkey="52" /label=Act\SAS /note="Actin splicing acceptor site. Accession m20310" CDS 2053..3089 /vntifkey="4" /label=Hygro\r /note="Hygromicin B phosphotransferase Accession k01193" 3'UTR 3112..3389 /vntifkey="50" /label=Act\3'\utr /note="Actin 3' untranslated region. Accession m20310" CDS 4335..5195 /vntifkey="4" /label=Amp\r /note="B-lactamase gene" promoter 942..961 /vntifkey="30" /label=T7\promoter\(10%) BASE COUNT 1491 a 1549 c 1606 g 1544 t ORIGIN 1 ctgaccgtat catgatgact ttctccatca tcccatcccc caaggtgtcc gacactgtcg 61 tcgagccgta caatacgact ctctccgtgc accaacttgt ggaaaactcc gatgagtcga 121 tgtgcattga caacgaggca ctgtacgata tttgcttccg caccctgaaa ctgacaacac 181 caacgttcgg tgacctgaac cacttggtgt ctgctgttgt gtccggcgtc acctgctgcc 241 tgcgcttccc tggtcagttg aactctgacc tccgtaagtt ggctgtgaac cttgtcccat 301 tcccgcgtct gcacttcttc atgatgggct tcgccccgct gaccagccgc ggctcgcagc 361 agtaccgcgg tctctccgtg cccgagctaa cgcagcagat gttcgatgcg aaaaacatga 421 tgcaagctgc agatcctcgt cacggccgct acctgacagc gtctgcactc ttccgcggcc 481 gcatgtcgac gaaggaggtt gatgagcaga tgctgaacgt gcagaacaag aactcgtcct 541 acttcattga gtggatcgat cccgaacaac atcaagtcct ctgtttgcga tatcccaccc 601 aagggactca agatggctgt caccttcatt ggcaacaaca cctgcatcca ggagatgttc 661 cgccgtgtgg gagagcagtt caccctcatg ttccgtcgca aggcgttctt gcactggtac 721 actggcgagg gtatggacga gatggaattc acggaggcag agtccaacat gaacgatctc 781 gtgtctgagt accagcagta ccaggatgcc acgattgagg aggagggcga gttcgacgag 841 gaggagcaat actagacgcg gacggggcat ttcccgttcg tcattagcag taggtaatga 901 agatgctcga gggtgctcaa gctgtgtagc gcacgcgccg gtaatacgtc tcactatagg 961 gagatctacc ggcgcgtttc cttacatatt tctctaacag gcacggaagc ctaacaaata 1021 cacttggctt atttttttgc cccctcatgt cttgtacaaa tatttgcgat agcttagcta 1081 tcagccacat taatcaaaca agtataccaa caagcccgaa aacataaact caactgcaac 1141 gaagctttca tatgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc 1201 ttaatgacgt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaga ctaggtgtag 1261 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca 1321 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag 1381 attttttacg taataaggct aaaagtttta gatgtgcttt actaagtcat cgcgatggag 1441 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat 1501 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg 1561 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag 1621 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat 1681 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat 1741 tagaaaaaca acttaaatgt gaaagtgggt cttaaggatc ctgcccattt agttggcttt 1801 tcccttgtct cgtgtctttt ccgtggaaag gttcccggag taatctgatg gcacagcagg 1861 gaggtgcgcc tgcaggttgg ttaggaaggg gggatgatgt aaaagaagaa aatgggggga 1921 ttcgagcccg ggcacagcaa ggtcttctga aattcatgtt tttttttttt ttactctgca 1981 ttgcagtctc cgctcttatt tagttttgct ttacgtaagg tctcgttgct gccataaaat 2041 aagctactag tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc 2101 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct 2161 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt 2221 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa 2281 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag 2341 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg 2401 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc 2461 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat 2521 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag 2581 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac 2641 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac 2701 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg 2761 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt 2821 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag 2881 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc 2941 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc 3001 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt 3061 ccgagggcaa aggaatagag tagatgccga ccgggatcga tcccccgatc ctaacaccgg 3121 gttgtgtggc caaaattgtt ctgtagtcgc tgtgagttga cacggctagt gcttatgatt 3181 ttcctcgcgt gtggtgcctg tactcagccc tatgccttat ttgcaacaca tttacgtaca 3241 gcgcacaaga gaagagaaga tcacttgaag ataataaata tagggttgta ggcatcttgt 3301 ttaactcaaa ttttctcgtc ttggtgtgtc gacatgattg aaatagtgcc accagttgtg 3361 tttgatgcgt ttgttatcta tgcagtattc tgcaaggcct tgcaggcatg caagctagct 3421 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg 3481 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag 3541 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc 3601 tgagtttctg gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa tcagcagggt 3661 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3721 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3781 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact 3841 gttgggcgcc atctccttgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta 3901 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata tggtgcactc 3961 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 4021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 4081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 4141 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 4201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 4261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 4321 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 4381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 4441 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 4501 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 4561 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 4621 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 4681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 4741 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 4801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 4861 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 4921 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 4981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 5041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 5101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 5161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 5221 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 5281 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 5341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 5401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 5461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 5521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 5641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 5701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 5761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 5821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 5881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 5941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 6001 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 6061 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 6121 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 6181 attaatgcag //