LOCUS pLEW79\cor 7150 bp DNA circular 2-JUN-2000
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|190025657|
COMMENT VNTDBDATE|196545660|
COMMENT VNTNAME|pLEW79 corrected|
COMMENT VNTAUTHORNAME|Tristan|
COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu|
COMMENT VNTOAUTHORNAME|Sigfried|
FEATURES Location/Qualifiers
rRNA complement(1..718)
/vntifkey="58"
/label=rRNA\spacer
CDS complement(999..1456)
/vntifkey="4"
/label=PhleoR
promoter 1623..1897
/vntifkey="29"
/label=PARP\promoter+\2\tet\operators
splicing_signal 1898..2041
/vntifkey="38"
/label=SAS\PARP
promoter complement(1592..1622)
/vntifkey="30"
/label=T7\promoter
splicing_signal 1476..1593
/vntifkey="38"
/label=SAS\actin
3'UTR 3920..4072
/vntifkey="50"
/label=Aldolase\3'\UTR
misc_feature 1882..1897
/vntifkey="21"
/label=TET\operator
misc_feature 1903..1918
/vntifkey="21"
/label=TET\operator
CDS 5295..6155
/vntifkey="4"
/label=AmpR
rep_origin 6720..7095
/vntifkey="33"
/label=E.\coli\ORI
CDS 2117..3766
/vntifkey="4"
/label=luciferase
BASE COUNT 1892 a 1678 c 1790 g 1790 t
ORIGIN
1 gaattcgagc tcatatagtt ggtatgtatt ctaattccag actactggcg tggataaaca
61 tgtcccctga ttaaaaggaa agattccata gcccattagt gcaaagataa ttggtacacc
121 gtcaaaaaca catgggagac cacacgatac gacactgtga ccgtagcatc aattccgcac
181 tcgatataac tctatcgaag aacttccatg gtacaaactg gtcgccacgg tctgctccac
241 acggagatca tcgtatcatt tttatcgata gcggccgcta tcgatgtatg ccttggccct
301 gatggcatgc caatttcact acaacggact atgtggaccc cgttatcatg gaaatgcgct
361 agttggagga agttagaccg cgccggaaaa gagaggggta gagaaaatga caacttggaa
421 gatatccaca cacgcacggt gaaacgttag caacaattat tagggaagca cgcttgccct
481 agtcccacga gtaaccaaga ctccaaaagc ctttctggca cagagagcga gccgaaatgg
541 aaaagagaaa caatgcctgc actaacacta ctgagcgatt cgcctcgccg cggaggaccg
601 aatactaata acgacacttg cggtcaaaaa gtagaagaac aaatgctcaa cgatgagtga
661 atcaggttag ggtagttgga aaattataca gaatgtcttt ggcaacacac cggctagctt
721 gcatgcctgc aaggccttgc agaatactgc atagataaca aacgcatcaa acacaactgg
781 tggcactatt tcaatcatgt cgacacacca agacgagaaa atttgagtta aacaagatgc
841 ctacaaccct atatttatta tcttcaagtg atcttctctt ctcttgtgcg ctgtacgtaa
901 atgtgttgca aataaggcat agggctgagt acaggcacca cacgcgagga aaatcataag
961 cactagccgt gtcaactcac agcgactaca gaacaatttt ggccacacaa cccggtgtta
1021 ggatctccga ggcctgggac ccgtgggccg ccgtcggacc ggcggtgttg gtcggcgtcg
1081 gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
1141 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
1201 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
1261 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
1321 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
1381 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
1441 tggtcaactt ggccatggtg atatagctta ttttatggca gcaacgagac cttacgtaaa
1501 gcaaaactaa ataagagcgg agactgcaat gcagagtaaa aaaaaaaaaa acatgaattt
1561 cagaagacct tgctgtgccc ccggtacggg ctagatctcc ctatagtgag tcgtattaat
1621 caggtaccgt cattggggtt aagcggaaag gtgtgtgtca gtaggttgtg aggtgaaagc
1681 gttttcagat gcatagtgag cttaatgtcc ttttcacagt atatcgtgtc tgataggtat
1741 ctcttattag tatagtcgaa tactagtcaa tagtgcgttt tgtgcaaaat gtccattttg
1801 tggcagtgat ggggttgttt tatgctattc cgtgtctctg ggtgggcgtg cattgaaaat
1861 aggggttatc gggtagggat ctccctatca gtgatagaga tctccctatc agtgatagag
1921 atccctgagt actgagttta acatgttctc gtcccgggct gcacgcgcct tcgagttttt
1981 tttccttttc cccatttttt tcaacttgaa gacttcaatt acaccaaaaa gtaaaattca
2041 caagcttgga attcctttgt gttacattct tgaatgtcgc tcgcagtgac attagcattc
2101 cggtactgtt ggtaaaatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
2161 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
2221 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
2281 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
2341 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
2401 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
2461 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
2521 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
2581 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
2641 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
2701 aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
2761 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
2821 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
2881 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg
2941 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt
3001 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
3061 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg
3121 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
3181 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
3241 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc
3301 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
3361 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt
3421 tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg ctgaattgga
3481 atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga
3541 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
3601 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
3661 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
3721 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa atgtaactgt
3781 attcagcgat gacgaaattc ttagctattg taatattata tgcaaattga tgaatggtaa
3841 ttttgtaatt gtgggtcact gtactatttt aacgaataat aaaatcaggt ataggtaact
3901 aaaaaggaat tcgagctcgg atcctgccca tttagttggc ttttcccttg tctcgtgtct
3961 tttccgtgga aaggttcccg gagtaatctg atggcacagc agggaggtgc gcctgcaggt
4021 tggttaggaa ggggggatga tgtaaaagaa gaaaatgggg ggattcgagc cccaattaac
4081 cctcactaaa gggagatctc ggagagagag agggtgggat ttttttcttt aaataaaggg
4141 agatgtattc ggaggagttg cattaacgtt gtcccatgtt ttacaaaaaa cgagtctgaa
4201 agaacgccga ctcaatgccc atcaagcgga ataaccttct aaaagaatca tatccctatt
4261 accacaccag tttatattac aggggaggtt attacagaaa tctaggatat cagactcaag
4321 gtgtggcgga cgtctcgaac cgatcgtgag ttggccccgt tgcaggcatg caagctagct
4381 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
4441 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
4501 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
4561 tgagtttctg gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa tcagcagggt
4621 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
4681 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
4741 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
4801 gttgggcgcc atctccttgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
4861 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata tggtgcactc
4921 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
4981 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
5041 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
5101 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
5161 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
5221 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
5281 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
5341 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
5401 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
5461 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
5521 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
5581 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
5641 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
5701 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
5761 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
5821 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
5881 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
5941 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
6001 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
6061 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
6121 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
6181 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
6241 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
6301 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
6361 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
6421 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
6481 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
6541 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
6601 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
6661 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
6721 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
6781 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6841 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
6901 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
6961 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
7021 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
7081 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
7141 attaatgcag
//