LOCUS pLEW100 7417 bp DNA circular 2-JUN-2000 SOURCE ORGANISM COMMENT CAn remove luciferase with HindIII and BamHI digestion. COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|196006586| COMMENT VNTDBDATE|196545832| COMMENT VNTNAME|pLEW100| COMMENT VNTAUTHORNAME|Tristan| COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu| COMMENT VNTOAUTHORNAME|Sigfried| FEATURES Location/Qualifiers rRNA complement(8..712) /vntifkey="58" /label=rRNA\spacer 3'UTR 719..1003 /vntifkey="50" /label=Actin\3'\UTR CDS complement(1004..1476) /vntifkey="4" /label=Ble 5'UTR 1477..1593 /vntifkey="52" /label=Actin\5'\SAS promoter complement(1599..1620) /vntifkey="30" /label=T7\promoter promoter 1629..1953 /vntifkey="29" /label=Tc-PARP\promoter /note="Tetracycline responsive PARP promoter xontaining 2 tandem tet operators" misc_feature 1903..1921 /vntifkey="21" /label=tet\operator misc_feature 1882..1899 /vntifkey="21" /label=tet\operator splicing_signal 1954..2042 /vntifkey="38" /label=PARP\SAS 3'UTR 3925..4630 /vntifkey="50" /label=Aldolase\long\3'\UTR\from\pHD103 misc_feature 4631..7 /vntifkey="21" /label=pGEM4 rep_origin 6987..7362 /vntifkey="33" /label=E.\coli\ori CDS 5562..6422 /vntifkey="4" /label=AmpR CDS 2117..3766 /vntifkey="4" /label=luciferase BASE COUNT 1950 a 1710 c 1859 g 1895 t 3 others ORIGIN 1 gaattcgagc tcatatagtt ggtatgtatt ctaattccag actactggcg tggataaaca 61 tgtcccctga ttaaaaggaa agattccata gcccattagt gcaaagataa ttggtacacc 121 gtcaaaaaca catgggagac cacacgatac gacactgtga ccgtagcatc aattccgcac 181 tcgatataac tctatcgaag aacttccatg gtacaaactg gtcgccacgg tctgctccac 241 acggagatca tcgtatcatt tttatcgata gcggccgcta tcgatgtatg ccttggccct 301 gatggcatgc caatttcact acaacggact atgtggaccc cgttatcatg gaaatgcgct 361 agttggagga agttagaccg cgccggaaaa gagaggggta gagaaaatga caacttggaa 421 gatatccaca cacgcacggt gaaacgttag caacaattat tagggaagca cgcttgccct 481 agtcccacga gtaaccaaga ctccaaaagc ctttctggca cagagagcga gccgaaatgg 541 aaaagagaaa caatgcctgc actaacacta ctgagcgatt cgcctcgccg cggaggaccg 601 aatactaata acgacacttg cggtcaaaaa gtagaagaac aaatgctcaa cgatgagtga 661 atcaggttag ggtagttgga aaattataca gaatgtcttt ggcaacacac cggcnnnctt 721 gcatgcctgc aaggccttgc agaatactgc atagataaca aacgcatcaa acacaactgg 781 tggcactatt tcaatcatgt cgacacacca agacgagaaa atttgagtta aacaagatgc 841 ctacaaccct atatttatta tcttcaagtg atcttctctt ctcttgtgcg ctgtacgtaa 901 atgtgttgca aataaggcat agggctgagt acaggcacca cacgcgagga aaatcataag 961 cactagccgt gtcaactcac agcgactaca gaacaatttt ggccacacaa cccggtgtta 1021 ggatctccga ggcctgggac ccgtgggccg ccgtcggacc ggcggtgttg gtcggcgtcg 1081 gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga 1141 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga 1201 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca 1261 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca 1321 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc 1381 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac 1441 tggtcaactt ggccatggtg atatagctta ttttatggca gcaacgagac cttacgtaaa 1501 gcaaaactaa ataagagcgg agactgcaat gcagagtaaa aaaaaaaaaa acatgaattt 1561 cagaagacct tgctgtgccc ccggtacggg ctagatctcc ctatagtgag tcgtattaat 1621 caggtaccgt cattggggtt aagcggaaag gtgtgtgtca gtaggttgtg aggtgaaagc 1681 gttttcagat gcatagtgag cttaatgtcc ttttcacagt atatcgtgtc tgataggtat 1741 ctcttattag tatagtcgaa tactagtcaa tagtgcgttt tgtgcaaaat gtccattttg 1801 tggcagtgat ggggttgttt tatgctattc cgtgtctctg ggtgggcgtg cattgaaaat 1861 aggggttatc gggtagggat ctccctatca gtgatagaga tctccctatc agtgatagag 1921 atccctgagt actgagttta acatgttctc gtcccgggct gcacgcgcct tcgagttttt 1981 tttccttttc cccatttttt tcaacttgaa gacttcaatt acaccaaaaa gtaaaattca 2041 caagcttgga attcctttgt gttacattct tgaatgtcgc tcgcagtgac attagcattc 2101 cggtactgtt ggtaaaatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta 2161 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct 2221 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga 2281 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa 2341 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc 2401 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct 2461 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa 2521 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa 2581 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg 2641 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat 2701 aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc 2761 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac 2821 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata 2881 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg 2941 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt 3001 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg 3061 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg 3121 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg 3181 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga 3241 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc 3301 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga 3361 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt 3421 tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg ctgaattgga 3481 atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga 3541 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga 3601 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg 3661 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat 3721 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa atgtaactgt 3781 attcagcgat gacgaaattc ttagctattg taatattata tgcaaattga tgaatggtaa 3841 ttttgtaatt gtgggtcact gtactatttt aacgaataat aaaatcaggt ataggtaact 3901 aaaaaggaat tcgagctcgg atcctgccca tttagttggc ttttcccttg tctcgtgtct 3961 tttccgtgga aaggttcccg gagtaatctg atggcacagc agggaggtgc gcctgcaggt 4021 tggttaggaa ggggggatga tgtaaaagaa gaaaatgggg ggatatctta tgtttaagag 4081 gataaataat gtgaaggggc tttatatgct tgcttggttg tctcgttggt gcatggggaa 4141 tctgcatgtt tgctttggag cacgcgtggt acatcaatgg gatcatatct tgctgcctcc 4201 cgcagctcac cgtgcgagct gccgggaccc cgtttctcat aggcgtgtgc accttccgtc 4261 tgagacctgt aaatggttaa aaaggaatat ataattacct tttgaaaagt ggtaaaaaac 4321 gaatatatct ttttttttgg ttttaatacg ctcttttgtg tgtatgagag gaataaataa 4381 gtgtgtgtgt gtgtgtgtgt tgttgttgtt gaaaaaatga aggacacgaa agcgctcatt 4441 aagggtgggg atacaccagg cataattatc cgaaaatatt ttgatgcacc aaataagtga 4501 acatactgac gaaatcaaag agttggagga tggataggga ggcctcattg gcagtggtaa 4561 attgatttga cttgaatatg ataccctctt actgtttcat tgccactaca agttggtttc 4621 cttcccctgc aggcatgcaa gctagcttgt attctatagt gtcacctaaa tcgtatgtgt 4681 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct 4741 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc 4801 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgttcc gcaccccgga 4861 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat 4921 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac 4981 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat 5041 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcacc attccttgcg 5101 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat 5161 aagggagagc gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 5221 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 5281 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 5341 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 5401 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 5461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 5521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 5581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 5641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 5701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 5761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 5821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 5881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 5941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 6001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 6061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 6121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 6181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 6241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 6301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 6361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 6421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 6481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 6541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 6601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 6661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 6721 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 6781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 6841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 6901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 6961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 7021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 7081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 7141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 7201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 7261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 7321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 7381 cgcctctccc cgcgcgttgg ccgattcatt aatgcag //