LOCUS pZJM 6371 bp DNA circular 23-OCT-2000
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|209392556|
COMMENT VNTDBDATE|209399221|
COMMENT VNTNAME|pZJM|
COMMENT VNTAUTHORNAME|Tristan|
COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu|
FEATURES Location/Qualifiers
rep_origin 5941..6316
/vntifkey="33"
/label=E.\coli\ORI
CDS 4516..5376
/vntifkey="4"
/label=AmpR
3'UTR 2879..3584
/vntifkey="50"
/label=Aldolase\3'\UTR
rRNA complement(8..712)
/vntifkey="58"
/label=rRNA\spacer
CDS complement(1004..1476)
/vntifkey="4"
/label=BleoR
3'UTR 719..1003
/vntifkey="50"
/label=Actin\3'\UTR
5'UTR 1477..1593
/vntifkey="52"
/label=Actin\5'\SAS
insertion_seq 1910..2573
/vntifkey="14"
/label=brucei\a-tubulin\frag
promoter complement(1599..1618)
/vntifkey="30"
/label=T7\promoter
promoter 1859..1878
/vntifkey="30"
/label=T7\promoter
promoter complement(2623..2642)
/vntifkey="30"
/label=T7\promoter
misc_feature 2599..2617
/vntifkey="21"
/label=Tet\operator
misc_feature 1884..1902
/vntifkey="21"
/label=Tet\operator
terminator 2745..2872
/vntifkey="43"
/label=T7\terminator
terminator 1629..1756
/vntifkey="43"
/label=T7\terminator
BASE COUNT 1578 a 1600 c 1651 g 1539 t 3 others
ORIGIN
1 gaattcgagc tcatatagtt ggtatgtatt ctaattccag actactggcg tggataaaca
61 tgtcccctga ttaaaaggaa agattccata gcccattagt gcaaagataa ttggtacacc
121 gtcaaaaaca catgggagac cacacgatac gacactgtga ccgtagcatc aattccgcac
181 tcgatataac tctatcgaag aacttccatg gtacaaactg gtcgccacgg tctgctccac
241 acggagatca tcgtatcatt tttatcgata gcggccgcta tcgatgtatg ccttggccct
301 gatggcatgc caatttcact acaacggact atgtggaccc cgttatcatg gaaatgcgct
361 agttggagga agttagaccg cgccggaaaa gagaggggta gagaaaatga caacttggaa
421 gatatccaca cacgcacggt gaaacgttag caacaattat tagggaagca cgcttgccct
481 agtcccacga gtaaccaaga ctccaaaagc ctttctggca cagagagcga gccgaaatgg
541 aaaagagaaa caatgcctgc actaacacta ctgagcgatt cgcctcgccg cggaggaccg
601 aatactaata acgacacttg cggtcaaaaa gtagaagaac aaatgctcaa cgatgagtga
661 atcaggttag ggtagttgga aaattataca gaatgtcttt ggcaacacac cggcnnnctt
721 gcatgcctgc aaggccttgc agaatactgc atagataaca aacgcatcaa acacaactgg
781 tggcactatt tcaatcatgt cgacacacca agacgagaaa atttgagtta aacaagatgc
841 ctacaaccct atatttatta tcttcaagtg atcttctctt ctcttgtgcg ctgtacgtaa
901 atgtgttgca aataaggcat agggctgagt acaggcacca cacgcgagga aaatcataag
961 cactagccgt gtcaactcac agcgactaca gaacaatttt ggccacacaa cccggtgtta
1021 ggatctccga ggcctgggac ccgtgggccg ccgtcggacc ggcggtgttg gtcggcgtcg
1081 gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
1141 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
1201 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
1261 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
1321 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
1381 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
1441 tggtcaactt ggccatggtg atatagctta ttttatggca gcaacgagac cttacgtaaa
1501 gcaaaactaa ataagagcgg agactgcaat gcagagtaaa aaaaaaaaaa acatgaattt
1561 cagaagacct tgctgtgccc ccggtacggg ctagatctcc ctatagtgag tcgtattaat
1621 caggtacccc ggatatagtt cctcctttca gcaaaaaacc cctcaagacc cgtttagagg
1681 ccccaagggg ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc
1741 gggctttgtt agcagcccta tccggatata gttctccttt cagcaaaaaa cccctcaaga
1801 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcgggctag cacctgatta
1861 atacgactca ctatagggag atctccctat cagtgataga gatctagccc ctcgagatgc
1921 gtgaggctat ctgcatccac attggtcagg ctggttgcca ggttggtaac gcctgctggg
1981 aattgttctg cctggaacac ggcattcaac ccgatggtgc gatgccctct gacaagacga
2041 ttggcgttga ggatgatgcg ttcaacacct tcttctctga gactggtgct ggcaagcacg
2101 ttccccgcgc ggtgttcttg gacctggagc caacagtggt ggatgaagtg cgcactggca
2161 cgtaccgcca gctgttccac cccgagcagc tgatctccgg caaggaggat gcggccaaca
2221 actacgctcg tggccactac accattggta aggagatcgt cgacctctgc ctggaccgca
2281 tccgcaagct cgctgacaac tgcactggtc ttcagggctt cctcgtgtat cacgccgtcg
2341 gcggtggcac tggttctggc ctgggtgcgc tgctcttgga gcgcctctcc gtggactatg
2401 gcaagaagtc caagctcggc tacacggtgt atccatcacc gcaggtgtca acggctgtcg
2461 tggagcccta caactctgtg ctctcgacac actcacttct ggagcacacc gatgttgctg
2521 cgatgcttga caatgaagca atttatgatt tgactcgccg caacctaagc ttgatatcga
2581 attcctgcag gggctagatc tctatcactg atagggagat ctccctatag tgagtcgtat
2641 taatcaggtg ctagcccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
2701 tcttgagggg ttttttgctg aaaggagaac tatatccgga tagggctgct aacaaagccc
2761 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
2821 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggggatcctg
2881 cccatttagt tggcttttcc cttgtctcgt gtcttttccg tggaaaggtt cccggagtaa
2941 tctgatggca cagcagggag gtgcgcctgc aggttggtta ggaagggggg atgatgtaaa
3001 agaagaaaat ggggggatat cttatgttta agaggataaa taatgtgaag gggctttata
3061 tgcttgcttg gttgtctcgt tggtgcatgg ggaatctgca tgtttgcttt ggagcacgcg
3121 tggtacatca atgggatcat atcttgctgc ctcccgcagc tcaccgtgcg agctgccggg
3181 accccgtttc tcataggcgt gtgcaccttc cgtctgagac ctgtaaatgg ttaaaaagga
3241 atatataatt accttttgaa aagtggtaaa aaacgaatat atcttttttt ttggttttaa
3301 tacgctcttt tgtgtgtatg agaggaataa ataagtgtgt gtgtgtgtgt gtgttgttgt
3361 tgttgaaaaa atgaaggaca cgaaagcgct cattaagggt ggggatacac caggcataat
3421 tatccgaaaa tattttgatg caccaaataa gtgaacatac tgacgaaatc aaagagttgg
3481 aggatggata gggaggcctc attggcagtg gtaaattgat ttgacttgaa tatgataccc
3541 tcttactgtt tcattgccac tacaagttgg tttccttccc ctgcaggcat gcaagctagc
3601 ttgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
3661 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
3721 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
3781 ctgagtttct ggtaacgccg ttccgcaccc cggaaatggt cagcgaacca atcagcaggg
3841 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
3901 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
3961 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
4021 tgttgggcgc catctccttg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
4081 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat atggtgcact
4141 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4201 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4261 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4321 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4381 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
4441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
4561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
4621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
4681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
4741 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
4801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
4861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
4921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
4981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5041 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
5401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
5461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
5521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
5581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
5641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
5701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
5761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
5821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
5881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
5941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
6241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
6301 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
6361 cattaatgca g
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