LOCUS pT7KO1c\revised 8584 bp DNA circular
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|185642028|
COMMENT VNTAUTHORNAME|Larry Simpson|
FEATURES Location/Qualifiers
CDS 6688..7548
/vntifkey="4"
/label=AmpR
/note="lactamase"
3'UTR 5484..6018
/vntifkey="50"
/label=p110\3'\UTR
/note="p110 3' UTR"
CDS 4139..5179
/vntifkey="4"
/label=HygroR
/note="Accession k01193"
5'UTR 497..784
/vntifkey="52"
/label=p110\5'\UTR
/note="5' UTR of T. brucei p110 gene"
CDS 1114..3798
/vntifkey="4"
/label=T7\RNA\polymerase
splicing_signal 988..1073
/vntifkey="38"
/label=PARP\SAS
BASE COUNT 2088 a 2132 c 2202 g 2162 t
ORIGIN
1 gaattcgagc tcggtaccag ctgaccgtat catgatgact ttctccatca tcccatcccc
61 caaggtgtcc gacactgtcg tcgagccgta caatacgact ctctccgtgc accaacttgt
121 ggaaaactcc gatgagtcga tgtgcattga caacgaggca ctgtacgata tttgcttccg
181 caccctgaaa ctgacaacac caacgttcgg tgacctgaac cacttggtgt ctgctgttgt
241 gtccggcgtc acctgctgcc tgcgcttccc tggtcagttg aactctgacc tccgtaagtt
301 ggctgtgaac cttgtcccat tcccgcgtct gcacttcttc atgatgggct tcgccccgct
361 gaccagccgc ggctcgcagc agtaccgcgg tctctccgtg cccgagctaa cgcagcagat
421 gttcgatgcg aaaaacatga tgcaagctgc agatcctcgt cacggccgct acctgacagc
481 gtctgcactc ttccgcggcc gcttagattc tgtcacctat tttgcaccct tttacctttt
541 ttctggtgtc ccatttctta agttggctgt aatcggcttt taaacctcaa ggcgctttga
601 aagaatagtt tacactttgt ttcctattga accattcgtt tagtactgtc aaaggaagca
661 aaggtgtgaa gaagaagaaa ggacaaaaaa aggacaaaaa aaccgaagaa gcgtattatt
721 tattcatcta tttatttatt agttagtgat accacgcaga ttcaatatcg aagcattacc
781 ggtctcgagg gtgctcaagc tgtgtagcgc acgcgtttcc ttacatattt ctctaacagg
841 cacggaagcc taacaaatac acttggctta tttttttgcc ccctcatgtc ttgtacaaat
901 atttgcgata gcttagctat cagccacatt aatcaaacaa gtataccaac aagcccgaaa
961 acataaactc aactgcaacg aagctgggct gcacgcgcct tcgagttttt tttccttttc
1021 cccatttttt tcaacttgaa gacttcaatt acaccaaaaa gtaaaattca caagctgatc
1081 tgatccggat ttactaactg gaagaggcac taaatgaaca cgattaacat cgctaagaac
1141 gaattcctcg agcctccaaa aaagaagaga aaggtcgaat tctctgacat cgaactggct
1201 gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg
1261 gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt
1321 caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta
1381 ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc
1441 aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc
1501 accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta
1561 gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt
1621 gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc
1681 tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt
1741 ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc
1801 gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta
1861 ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt
1921 gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg
1981 tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg
2041 cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac
2101 aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc
2161 gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt
2221 gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg
2281 aaacgtgctg ccgctgctgt gtaccgcaag gacagggctc gcaagtctcg ccgtatcagc
2341 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct
2401 tacaacatgg actggcgcgg tcgtgtttac gccgtgtcaa tgttcaaccc gcaaggtaac
2461 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac
2521 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag
2581 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg
2641 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag
2701 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac
2761 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc
2821 gcggttaact tgcttcctag tgagaccgtt caggacatct acgggattgt tgctaagaaa
2881 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg
2941 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct
3001 ggtcaatggc tggctcacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg
3061 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca
3121 gctattgatt ccggcaaggg tccgatgttc actcagccga atcaggctgc tggatacatg
3181 gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac
3241 tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag
3301 attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag
3361 gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta
3421 cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt
3481 atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg
3541 gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt
3601 ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag
3661 tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa
3721 ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag
3781 tcggacttcg cgttcgcgta acgccaaatc aatacgactc actatagagg gacaaactca
3841 aggtcattcg caagagtggc cggatcctgc ccatttagtt ggcttttccc ttgtctcgtg
3901 tcttttccgt ggaaaggttc ccggagtaat ctgatggcac agcagggagg tgcgcctgca
3961 ggttggttag gaagggggga tgatgtaaaa gaagaaaatg gggggattcg agcccgggca
4021 cagcaaggtc ttctgaaatt catgtttttt ttttttttac tctgcattgc agtctccgct
4081 cttatttagt tttgctttac gtaaggtctc gttgctgcca taaaataagc tactagtgat
4141 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
4201 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
4261 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
4321 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
4381 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
4441 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
4501 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
4561 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
4621 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
4681 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
4741 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
4801 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
4861 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
4921 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
4981 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
5041 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
5101 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
5161 atagagtaga tgccgaccgg gatcgatccc ccgatcctaa caccgggttg tgtggccaaa
5221 attgttctgt agtcgctgtg agttgacacg gctagtgctt atgattttcc tcgcgtgtgg
5281 tgcctgtact cagccctatg ccttatttgc aacacattta cgtacagcgc acaagagaag
5341 agaagatcac ttgaagataa taaatatagg gttgtaggca tcttgtttaa ctcaaatttt
5401 ctcgtcttgg tgtgtcgaca tgattgaaat agtgccacca gttgtgtttg atgcgtttgt
5461 tatctatgca gtattctgca aggccttgcg tttgtaacca actgtaaata tgtatagaaa
5521 atatccatgc atatttattt attttaataa ttaaattatg gtttatgcat gtaataatgc
5581 agtgaaacat gaagagatga aattgtattg gtatatgggc cctggttttc aggtaacaga
5641 atctgtacgc gtgcttattg ggttactttt ccttcttttc tcccgtttcc ctttctcttt
5701 tagtgatggt gctaacaata attataaata tatttatatt tctctacttt attccgtctt
5761 ccatctgatc attatgttca ggtgtaggca ttagggaagg gcgggtgatg tgatgtgtgt
5821 ggtgtgtatg tggggaagac aacacataaa tttcatttgt gcatttcccc ctccccccct
5881 ctccttcttc ctcctttctt tccttcgaaa tctgcagaaa caaaaaaata atggttatga
5941 tgacgatacg gaagaagacg gaagggtgga tgcactggta gaagtgaaag tagtgggaaa
6001 gggggagaag cggccgcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca
6061 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
6121 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
6181 tggccggggg actgttgggc gccatctcct tgcaccattc cttgcggcgg cggtgctcaa
6241 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
6301 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
6361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
6421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
6481 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
6541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
6601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
6661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
6721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
6781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
6841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
6901 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
6961 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
7021 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
7081 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
7141 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
7201 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
7261 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
7321 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
7381 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
7441 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
7501 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
7561 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
7621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
7681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
7741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
7801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
7861 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
7921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
7981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
8041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
8101 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
8161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
8221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
8281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
8341 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
8401 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
8461 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
8521 cgttggccga ttcattaatg cagctggctt atcgaaatta atacgactca ctatagggag
8581 accg
//