LOCUS pSNSAP1 8300 bp DNA circular 8-JUN-2005 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|372437634| COMMENT VNTDBDATE|376756024| COMMENT LSOWNER| COMMENT VNTNAME|pSNSAP1| COMMENT VNTAUTHORNAME|Saturn1| COMMENT VNTREPLTYPE|Plasmid FEATURES Location/Qualifiers CDS 896..1756 /vntifkey="4" /label=Amp\r CDS complement(2321..2696) /vntifkey="4" /label=E.\coli\ori CDS 8261..8284 /vntifkey="4" /label=Flag\tag misc_feature 7817..7837 /vntifkey="21" /label=TEV\cleavage\site /note="TEV cleavege site" misc_feature 6119..7648 /vntifkey="21" /label=LmRET2 /note="Ret2 polypeptide portion of tagged ORF" misc_feature 7655..7792 /vntifkey="21" /label=Strepavidin\binding\peptide\(SBP) /note="Streptavidin Binding Peptide" misc_feature 7793..7810 /vntifkey="21" /label=Thrombin\cleavage\site /note="Thrombin cleavage site" CDS 3893..4684 /vntifkey="4" /label=NeoR misc_feature 7844..8254 /vntifkey="21" /label=Protein\A BASE COUNT 1884 a 2354 c 2290 g 1772 t ORIGIN 1 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt 61 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 121 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cactggcgta 241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 301 ggcgcgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 361 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 421 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 481 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 541 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 601 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 661 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 721 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccaggtg gcacttttcg 781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 1021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 1081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 1141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 1201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 1261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 1321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 1381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 1441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 1501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 1561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 1621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 1681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 1741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 1801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 1861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 2581 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 2641 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 2701 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcgaaa 2761 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 2821 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg 2881 ccccccctcg aggtcgacgg ggtgatggag agaatggttg accatgagga cgccgatggg 2941 cgatgcgcca gcgcgtgctt gcgtacttca atcgtttttc atcttgattc agagctaagt 3001 agtagcacac cacagcaagc gtaatcatgt agcatgtatg caggtgcacg agtaggcact 3061 ctctgtctct gtccggcatc tatgatgctc tccggccgcg tgcgcgcgac gcctctgtgt 3121 gagtgctcgt gtgagtgtgt gagagctgtc caggcctttc ctctgcctac tcgtcccacg 3181 ctcaggcagg cacagaggaa tgaaaaggaa gacgaggacg caacgccagg cgcatcgaca 3241 tcgcgcacgt gtttgcggtg tactgtgcgt cgatgatcga gcggccaagc gtccagcttc 3301 acgcgaggag gactgcatcg ctgctcctgt gcgcgtgcgt gcacgcgtca aggcacgctc 3361 gattcgttcc cagggtggat accctccccc ctcccccagc tgcagacaac ccccaccctc 3421 tcgaggcttg cacccaggct cgtcagtctc tctaccactt tctgccttct gtagtgcacc 3481 ccacccctgc attctcctct ctctcacaca cacacacaca cacacacaca cacacacaca 3541 cacacgtaca cacgtacaca cacacgtgcg catgcttgac gcatacgcga cgaattcgaa 3601 agctcacctc attcctccct cctcacacca tcatcggcat ccatagagac acgtgcgcgt 3661 agaacgatac agctcacgcg cacagagagg cgtgttctgg tcgtgcgcat catcggcaca 3721 gcactggcgg acgaaactcg cacacaggca cgccgcctcc tttcacccgt catagatagt 3781 tgaattagac gccctcctcc tccctcatca tcgccgtcgt catccgggtc cgagcactag 3841 tcgcgtgtgg cctcgaacac cgagcgaccc tgcagccaat atgggatcgg ccattgaaca 3901 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3961 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 4021 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 4081 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 4141 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 4201 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 4261 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4321 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4381 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4441 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4501 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4561 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4621 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4681 ctgaggggat cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttcg 4741 gatcactagt gatcccgccc gcacccgcgc gcgctcaggc cgcgtgtcgt cgtgctcttt 4801 ccattttttt tttgtgtgtg gtggtgattt gagctgctct ccgttgtgtg ctggggaccc 4861 tccttccctc gatctcctcg tgcggggtct ccgacgcgca gacgcggtgc gtgcgagcgt 4921 gcacgtgctg tcgccgtggc tgtagttgcg agcggagaga gagagagagg gagggaggga 4981 gggcagaggg cagaggacat gcgggtggga acgtgcaccg gcctcgtctc acgcagctgg 5041 agcccacgaa tgccaccacc acacccttct tccccccccc tccctcttct tcccacggcg 5101 gcgacgacga cgggggctca gctcacgctt ctgtagggta ttatattaaa gcacatgtgc 5161 gtgctgtgct tcggcttctt gttggtggtc gttcgctttc aagtccacga ctcctgccgt 5221 tgctcaccgc cgggctgtct tctttcgccc ctccctcggt gcctcttccg ccgccgcacg 5281 cgtgcgctga tcacgcgtct tgcgtgatgt gcctgtgtgt atacacaccg tgcacggaga 5341 gagcgagcga gcgagagaga gagagggcga gaggaagaat ggccggtgcc tcgcgtgggc 5401 aagcgtgcgc gagtttgtcg tgtcgctgcg ccgcgtggat gtcgcgcagc gcaagacccc 5461 gtgcgcctga aacgtgaagg gaggtcgaga agcgtgccgc atgaggcctt aaggcaggaa 5521 agtgaaaaga gctcgacgcg agtgcagcac gcgcgacatc gaacaggacg gaatagacac 5581 tcagcccctt cccctttcaa aaactgaatg gacggacatc gtaacgcgct tctctcccct 5641 ccactccccc tgtccctgtc tccttgacgt gtgctgctct cgtgagcgac agatgaagcg 5701 aggcgtagaa ggaaaggggg gggagcgagg cctgcacaca cacacacaca cacacacaca 5761 cacacacaca cacacacaca cacacacaca cgaaaagcaa gtttgaggca gacgtggttt 5821 cacacgattc ctgtgcaaga agcgtgcctg cgtgacggtg tggctgtgta tgcatgcgtg 5881 aagggttacc gcaacgatgc gcaatggact cccccgcttt ccatttcgtc accttccgcc 5941 tctctctctc tctctctcac catctacgcg tgcacatcat caactgtctc ttgtcggtgc 6001 tcaccaccct caaccacccc tcactttcaa ggcttcccga acgcacacaa aaggcgtgaa 6061 aaccgctcgc gtgtgttgag ccgtccaccg tagccctccc cctgtccccg ggggatccat 6121 gcacgctcct acgccactgt acctctacgt ggccgtcacg cgcgggttgt gttccagcgc 6181 ggtggcggcg tcgagctcgg ctgacgctgc atcttcgccg agcgcggcgt cctcgtcgac 6241 gtccgagggg gtcagctatg cctcgcacct cgttgcttcg aacgatcact acgtacagtg 6301 gggtcgtgca ctgctggagg agaacagcaa gcggatcggt ccagagcaga tgtttcgtac 6361 ggccattcgg gcccaacagc agctgcagat gcttgccgac aagtggacac cggatgcgaa 6421 aatttactgc tgcgggtcgt tggtaacgca cgggcagatg gagtggggca gcgacctcga 6481 ccttgcctgt gtcttcgacg acccgtaccc cgaccactcc gtgcaggcga agcgcacgga 6541 aaagctgtgg tcagttgtca agcgctacat gccgcagttt ctgcgcaaca atctcttggc 6601 cctcacggag gctcgcaccc ctgtcgtgaa actgcgctac gtcaacgacg acaaggtcgc 6661 gcgggggcgg tacaccgtgc tgacggagga ggaggaccgc aagtcgcgca cgaccctgtg 6721 ggacatacgc aaccgccatc tggattccaa ggacctggag tacatcgcca caaagatggg 6781 gcgagagaat atcgagggca tctgggtgga gcggacgacg tatggctgcc ggtgcgcggt 6841 gcagtgcacg tcgcgcgagc aagccgtcgg ggctgtcggc ttcttcccag atggggtgat 6901 catgaccaag ggcatgcgcg aggactacac gcgcgacatg ctggacctcc gatacgtgcc 6961 ggagatgttt atgtacaagt gggatatttc ttttgtggct tacggcgtca agaacagcta 7021 cctgctccgc cactatctgc acaacggccc cccagctgcc cgccacgcag ccatggccgc 7081 gaaggcgtgg ggcaaggcct ccggcgtggg gctggggacg gcagccatgc tcacctccta 7141 cgcggtgacg gtcatgtttg ttcactacct cttggcaact cggcagctgg cctggatcga 7201 cccgtggtcg ttagcccatc cagtccacat gccgcgcttc cccgacttct ccccactttt 7261 ggactgcgac ccggccgagc tcggcaagct cctgcacggc ttcttcatct actacgcgtc 7321 tcacttcaac tatgagaaga acattatcag cctcagccgc ccacggcttt cactccgctc 7381 cgacgtgaac tggaacttcc cgcagagcaa gcagggcaca ttcagctacc tcctcggcat 7441 cgaggacccg tacgaagacg tcggggttgg cggactgaac ctcggtcgcc acctgcatcc 7501 agctaagttc aaccaggtga agcaggaatt cgcgcgtgcg gccaccacga tggagcgcta 7561 cgtgccgtcg aatgcgcccg aaaagacaat cctcggtgtc agacgcgggg gtacgtcgaa 7621 ccaccatcat caccataacc gcttcgattc tagaatggac gagaagacca ccggctggcg 7681 gggcggccac gtggtggagg gcctggccgg cgagctggag cagctgcggg ccaggctgga 7741 gcaccaccct cagggccagc gggagccctc cggcggctgc aagctgggcc tgctggttcc 7801 gcgtggatct gctagcgaga atttgtattt tcagggtgag ctcaaaaccg cggctcttgc 7861 gcaacacgat gaagccgtgg acaacaaatt caacaaagaa caacaaaacg cgttctatga 7921 gatcttacat ttacctaact taaacgaaga acaacgaaac gccttcatcc aaagtttaaa 7981 agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgctca 8041 ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt 8101 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga 8161 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatggtg ctcaggcgcc 8221 gaaagtagac gcgaattccg cggggaagtc aacccaattg gattataaag atgatgatga 8281 taaataacta gagcggccgc //