LOCUS pEKA2-revised 8355 bp DNA circular FEATURES Location/Qualifiers rep_origin complement(7924..8300) /vntifkey="33" /label=ORI /note="E. coli pMB2 replication origin" CDS 2..785 /vntifkey="4" /label=LtHGPRT\ORF\+\3'\UTR splicing_signal 786..956 /vntifkey="38" /label=Aldolase\5'\UTR\SAS splicing_signal 962..1047 /vntifkey="38" /label=PARP\SAS /note="Accession S60066. There is SAS redundancy due to need for site during cloning." 3'UTR 3941..4095 /vntifkey="50" /label=3'\aldolase\UTR /note="Accession X52586" CDS 4221..5261 /vntifkey="4" /label=HygroR /note="Accession K01193" 3'UTR 5277..5554 /vntifkey="50" /label=Actin\3'\UTR /note="Accession M20310" CDS 6500..7360 /vntifkey="4" /label=AmpR CDS 1192..3876 /vntifkey="4" /label=T7\RNA\polymerase BASE COUNT 2013 a 2122 c 2213 g 2007 t ORIGIN 1 ctgatgagca actcagtcaa gtccccttcc gaccatgtcg gtgatgtggg gcggcgcaac 61 tacccgatgt cggcccgcac gctcgtcaca caggagcagg tgtgggccgc cacagcgaag 121 tgcgcaaaga agattgcagc ggactacaag gacttccact taacggccga caacccgctc 181 tacttgctgt gcgtgctcaa gggcagcttc atcttcacgg ccgacctcgc ccgctttctg 241 gccgacgagg gggtgcctgt gaaggtggag tttatctgcg ctagctcgta cggctcgggc 301 gtggagacgt cgggtcaggt gcgcatgctt ctcgacgtgc gcgactccgt ggaaaaccgc 361 catattatgc ttgtcgagga catagtcgac agcgccatca cactgcagta cctgatgcgg 421 ttcatgctcg ccaagaagcc cgcctcgctg aagaccgtag tacttctgga caagccgtcg 481 gggcgcaagg tggatgtgct ggttgactac cccgtcatca cgatcccaca cgcgtttgtg 541 atcggctacg tatggacctt cgccgatcta cggggagtgc ctgatatctg cgttctaaag 601 aaggaatact atgagaaggc ggagagcaag ctgtagtggc gacgcgttac cactcgtgct 661 ggcgggcaag tgcgccgctc tctttcctcg tgaatgccct tttcacggac ccccccacac 721 acacgtacag ggacatgcta acaagcatgc acgtggccgg tgaaagtgac gaggctgcag 781 tgacgcgcgt ttccttacat atttctctaa caggcacgga agcctaacaa atacacttgg 841 cttatttttt tgccccctca tgtcttgtac aaatatttgc gatagcttag ctatcagcca 901 cattaatcaa acaagtatac caacaagccc gaaaacataa actcaactgc aacgaagctg 961 ggctgcacgc gccttcgagt tttttttcct tttccccatt tttttcaact tgaagacttc 1021 aattacacca aaaagtaaaa ttcacaagct gatctgatcc ggatttacta actggaagag 1081 gcactaaatg aacacgatta acatcgctaa gaacgaattc ctcgagcctc caaaaaagaa 1141 gagaaaggtc gaattctctg atccggattt actaactgga agaggcacta aatgaacacg 1201 attaacatcg ctaagaacga attcctcgag cctccaaaaa agaagagaaa ggtcgaattc 1261 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt 1321 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc 1381 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag 1441 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa 1501 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg 1561 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat 1621 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc 1681 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac 1741 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc 1801 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat 1861 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc 1921 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct 1981 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc 2041 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt 2101 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt 2161 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac 2221 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac 2281 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct 2341 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga cagggctcgc 2401 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat 2461 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc cgtgtcaatg 2521 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca 2581 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat 2641 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct 2701 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc 2761 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc 2821 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga 2881 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg agaccgttca ggacatctac 2941 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat 3001 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg 3061 ggcactaagg cactggctgg tcaatggctg gctcacggtg ttactcgcag tgtgactaag 3121 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg 3181 gaagatacca ttcagccagc tattgattcc ggcaagggtc cgatgttcac tcagccgaat 3241 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct 3301 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa 3361 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat 3421 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc 3481 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca 3541 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt 3601 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac 3661 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact 3721 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac 3781 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac 3841 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaac gccaaatcaa tacgactcac 3901 tatagaggga caaactcaag gtcattcgca agagtggccg gatcctgccc atttagttag 3961 ttggcttttc ccttgtctcg tgtcttttcc gtggaaaggt tcccggagta atctgatggc 4021 acagcaggga ggtgcgcctg caggttggtt aggaaggggg gatgatgtaa aagaagaaaa 4081 tggggggatt cgagcccggg cacagcaagg tcttctgaaa ttcatgtttt tttttttttt 4141 actctgcatt gcagtctccg ctcttattta gttttgcttt acgtaaggtc tcgttgctgc 4201 cataaaataa gctactagtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt 4261 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat 4321 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg 4381 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga 4441 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc 4501 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc 4561 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg 4621 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga 4681 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg 4741 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc 4801 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg 4861 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct 4921 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc 4981 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac 5041 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg 5101 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg 5161 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca 5221 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaaggat cgatcctaac 5281 accgggttgt gtggccaaaa ttgttctgta gtcgctgtga gttgacacgg ctagtgctta 5341 tgattttcct cgcgtgtggt gcctgtactc agccctatgc cttatttgca acacatttac 5401 gtacagcgca caagagaaga gaagatcact tgaagataat aaatataggg ttgtaggcat 5461 cttgtttaac tcaaattttc tcgtcttggt gtgtcgacat gattgaaata gtgccaccag 5521 ttgtgtttga tgcgtttgtt atctatgcag tattctgcaa ggccttgcag gcatgcaagc 5581 tagcttgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt 5641 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac 5701 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa 5761 ccttctgagt ttctggtaac gccgttccgc accccggaaa tggtcagcga accaatcagc 5821 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc 5881 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc 5941 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg 6001 ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc aacggcctca 6061 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatatggtg 6121 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 6181 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 6241 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 6301 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 6361 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 6421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 6481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 6541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 6601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 6661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 6721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 6781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 6841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 6901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 6961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 7021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 7081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 7141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 7201 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 7261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 7321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 7381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 7441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 7501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 7561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 7621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 7681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 7741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 7801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 7861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 7921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 7981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 8041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 8101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 8161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 8221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 8281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 8341 gattcattaa tgcag //